Biosynthesis and characterization of polyhydroxyalkanoates in the polysaccharide-degrading marine bacterium Saccharophagus degradans ATCC 43961

Journal of Industrial Microbiology & Biotechnology
Yolanda González-GarcíaGerhart Braunegg

Abstract

The marine bacterium Saccharophagus degradans was investigated for the synthesis of polyhydroxyalkanoates (PHAs), using glucose as the sole source of carbon in a two-step batch culture. In the first step the microorganism grew under nutrient balanced conditions; in the second step the cells were cultivated under limitation of nitrogen source. The biopolymer accumulated in S. degradans cells was detected by Nile red staining and FT-IR analysis. From GC-MS analysis, it was found that this strain produced a homopolymer of 3-hydroxybutyric acid. The cellular polymer concentration, its molecular mass, glass transition temperature, melting point and heat of fusion were 17.2+/-2.7% of dry cell weight, 54.2+/-0.6 kDa, 37.4+/-6.0 degrees C, 165.6+/-5.5 degrees C and 59.6+/-2.2 J g(-1), respectively. This work is the first report determining the capacity of S. degradans to synthesize PHAs.

References

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Citations

Feb 18, 2011·Journal of Industrial Microbiology & Biotechnology·Haitao ZhangSteven W Hutcheson
May 18, 2016·New Biotechnology·Martin KollerGerhart Braunegg
Nov 9, 2016·Critical Reviews in Microbiology·Ganesan SathiyanarayananJoseph Selvin
Sep 28, 2017·Bioengineering·Constantina KourmentzaMaria A M Reis

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