PMID: 11332893May 3, 2001Paper

Biosynthesis and photodynamic efficacy of protoporphyrin IX (PpIX) generated by 5-aminolevulinic acid (ALA) or its hexylester (hALA) in rat bladder carcinoma cells

Journal of Photochemistry and Photobiology. B, Biology
Isabelle Cosserat-GerardinFrançois Guillemin

Abstract

Hexylester of 5-aminolevulinic acid (hALA) has been considered as an alternative to 5-aminolevulinic acid (ALA) for the treatment of malignancies of different origin. The present study addresses the ALA and hALA-induced PpIX pharmacokinetic profile using rat bladder carcinoma cells (AY27). The total PpIX content measured spectrofluorimetrically after extraction procedure at the end of 2 h incubation was at least 1.5-fold greater with hALA compared to ALA despite the difference in concentration of several orders between the two compounds (1 or 5 mM ALA and 5 or 10 x 10(-3) mM hALA). Considerable PpIX efflux was detected in the extracellular medium at the end of the incubation. With 5 mM ALA and 10 x 10(-3) mM hALA, PpIX build-up was continued beyond the incubation period pointing out to enzyme saturation in the biosynthetic pathway or/and the constitution of ALA reserve. Red laser light (lambda=630 nm) irradiation of AY27 cells after 2 h incubation with increasing ALA or hALA concentrations resulted in a nearly equal photocytotoxicity.

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Citations

Nov 29, 2013·Photodiagnosis and Photodynamic Therapy·Asta JuzenieneJohan Moan
Nov 14, 2002·European Urology·Marie-Ange D'HallewinFrançois Guillemin
May 15, 2003·Journal of Photochemistry and Photobiology. B, Biology·R F TurchielloA C Tedesco
Jun 1, 2005·Photodiagnosis and Photodynamic Therapy·Miron KaliszewskiJohan Moan
Dec 27, 2007·Photomedicine and Laser Surgery·Maria Wołuń-CholewaWojcieh Warchoł
Apr 14, 2009·Journal of Photochemistry and Photobiology. B, Biology·M C M GrimbergenN Stone
Sep 2, 2003·Lasers in Surgery and Medicine·Johanna T H M van den AkkerStanley B Brown
Mar 21, 2006·The International Journal of Biochemistry & Cell Biology·Sinan BattahAlexander J MacRobert
Nov 24, 2005·Cancer Letters·Constance Lay Lay SawPaul Wan Sia Heng
Jan 1, 2016·Zhurnal voprosy neĭrokhirurgii imeni N. N. Burdenko·V A ShurkhayA A Potapov
May 26, 2017·PloS One·María Julia LambertiViviana Alicia Rivarola
Nov 2, 2019·MedChemComm·Jorge Peiró CadahíaMads H Clausen
Apr 17, 2019·Bioorganic & Medicinal Chemistry Letters·Moses BioYoungjae You

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