Biosynthesis and regulation of expression of the HNK-1 epitope on myelin-associated glycoprotein in a transfected cell model system

Journal of Neuroscience Research
L PedrazaJ L Salzer

Abstract

The HNK-1 antibody recognizes a carbohydrate epitope expressed by many cell adhesion molecules in the nervous system that has been proposed to be an important adhesive determinant. This epitope is particularly prominent on the myelin-associated glycoprotein (MAG) and is related to the antigenic target in an autoimmune mediated demyelinating neuropathy. Elucidation of the mechanisms underlying the biosynthesis and regulation of expression of the HNK-1 epitope is therefore likely to have important functional and clinical implications. In order to investigate its biosynthesis and the regulation of its expression, we have expressed both human and rat MAG in several different cell lines by retroviral infection. These studies indicate that the cellular milieu determines whether the HNK-1 epitope is expressed on the MAG polypeptide and provide an explanation for the significant variation in HNK-1 levels that has been noted in different species. Using a transfected human neuroblastoma line, we have determined that this epitope is present on the fourth and/or fifth immunoglobulin-like domain of rat MAG and that it is added intracellularly, probably in the trans Golgi. Finally we have found that expression of the HNK-1 epitope is increas...Continue Reading

References

Mar 6, 1992·Cell·I Mellman, K Simons
Jan 1, 1990·Journal of Neuroimmunology·J SrinivasanN Latov
Aug 2, 1990·Nature·T A Springer
Jan 1, 1990·Annual Review of Neuroscience·T M JessellJ Dodd
Jun 1, 1990·Journal of Neurochemistry·P S MohanF B Jungalwala
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M ArquintR Dunn
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C LaiJ G Sutcliffe
Jan 1, 1988·The Journal of Cell Biology·V KünemundM Schachner
Sep 29, 1989·Biochemical and Biophysical Research Communications·S SatoT Miyatake
Oct 1, 1989·Journal of Neuroscience Research·G SpagnolN Latov
May 8, 1989·Brain Research·M A MenaD Slonim
Jan 1, 1989·Developmental Neuroscience·J L Salzer, D R Colman
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S PorterR P Bunge
Oct 1, 1987·Developmental Biology·M Bronner-Fraser
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K B SeamonJ W Daly
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Hoffman, G M Edelman
Dec 15, 1993·Biochemical and Biophysical Research Communications·D BurgerA J Steck

❮ Previous
Next ❯

Citations

Feb 1, 1997·Journal of Molecular Neuroscience : MN·R H Quarles
May 20, 1998·Molecular Neurobiology·K C BreenF D Hayes
Nov 9, 2000·Progress in Retinal and Eye Research·M Uusitalo, T Kivelä
Jan 13, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yves AlloryHanna Debiec

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.