PMID: 6983958Dec 15, 1982Paper

Biosynthesis and secretion of alpha 1-antitrypsin in primary cultures of rat hepatocytes. Characterization of differently glycosylated intracellular and extracellular forms

European Journal of Biochemistry
V GrossP C Heinrich

Abstract

The biosynthesis and secretion of alpha 1-antitrypsin was studied in rat hepatocyte primary cultures. After labeling with [35S]methionine an alpha 1-antitrypsin with an apparent molecular weight of 49000 estimated by sodium dodecyl sulfate/polyacrylamide slab gel electrophoresis was immunoprecipitated from the cell homogenate. This intracellular form of alpha 1-antitrypsin could be deglycosylated by endoglycosidase H treatment indicating that its oligosaccharide chains were of the high-mannose type. Pulse-chase experiments showed that about 30 min after its synthesis the transformation of the 49000-Mr alpha 1-antitrypsin to a protein with an apparent molecular weight of 54000 began. Only this 54000-Mr protein was secreted by the hepatocytes. The 54000-Mr alpha 1-antitrypsin was not sensitive to endoglycosidase H, but sensitive to neuraminidase, and it incorporated [3H]galactose and [3H]fucose indicating that its oligosaccharide chains were of the complex type. In the presence of tunicamycin, which blocks the formation of N-asparagine-linked oligosaccharide chains, an unglycosylated alpha 1-antitrypsin with an apparent molecular weight of 41000 was found in the cells as well as in the medium. However, tunicamycin decreased the s...Continue Reading

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Citations

Jan 1, 1983·Developmental Biology·J M Icardo, F J Manasek
Jul 1, 1983·European Journal of Biochemistry·T AndusP C Heinrich
Jul 15, 1983·European Journal of Biochemistry·A EisenmannP C Heinrich
Jan 15, 1985·European Journal of Biochemistry·J BauerP C Heinrich
Dec 16, 1985·European Journal of Biochemistry·P VischerB Voss
Jan 2, 1987·European Journal of Biochemistry·V GrossW Gerok
Mar 1, 1984·Experimental Cell Research·V GrossP C Heinrich
May 19, 2010·Traffic·Veronika ReitererHans-Peter Hauri
Feb 20, 2008·The Journal of Cell Biology·Beat NyfelerHans-Peter Hauri
Mar 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J CarlsonT Kjellström
Jul 1, 1985·The British Journal of Nutrition·E C LewisJ Coppes
Jan 16, 1985·Biochemical and Biophysical Research Communications·W WeberP C Heinrich

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