PMID: 2118964Oct 1, 1990Paper

Biosynthesis, maturation, and acid activation of the Semliki Forest virus fusion protein

Journal of Virology
M KielianS DeCandido

Abstract

The Semliki Forest virus spike protein has a potent membrane fusion activity which is activated in vivo by the low pH of endocytic vacuoles. The spike protein is composed of two transmembrane subunits, E1 and E2, plus E3, a peripheral polypeptide. Acid-induced conformational changes in the E1 or E2 subunits were analyzed by using monoclonal antibodies specific for the acid-treated spike protein. E1 and E2 reacted with the antibodies after treatment of wild-type or mutant virus at the pH of fusion. The E1 conformational change resembled fusion in its requirement for both low pH and cholesterol. Pulse-chase analysis and intracellular pH treatment were then used to determine the ability of the newly synthesized spike to undergo acid-induced conformational changes. p62, the precursor to E2 and E3, was shown to undergo a pH-dependent conformational change similar to that of E2 and was sensitive to acid very soon after biosynthesis. In contrast, a posttranslational maturation event was required for the conversion of E1 to the pH-sensitive form. E1 maturation occurred fairly late in the exocytic pathway, after the virus spike had passed the medial Golgi but before incorporation of the spike into a new virus particle.

References

Aug 1, 1975·Journal of Virology·S Keränen, L Kääriäinen
Dec 1, 1987·Analytical Biochemistry·K A Giuliano, R J Gillies
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I de Curtis, K Simons
Jan 1, 1987·Annual Review of Biochemistry·E A Dawidowicz
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Dec 1, 1985·The Journal of Cell Biology·M Kielian, A Helenius
Feb 1, 1984·The Journal of Cell Biology·L TanasugarnD L Taylor
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M Chow, D Baltimore
May 1, 1983·Quarterly Reviews of Biophysics·J WhiteA Helenius
Jan 1, 1984·Advances in Protein Chemistry·K Simons, G Warren
Oct 1, 1984·Journal of Virology·M C Kielian, A Helenius
Jan 1, 1984·The Journal of Cell Biology·M C KielianA Helenius
Feb 1, 1980·The Journal of Cell Biology·A HeleniusE Fries
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J White, A Helenius
Jan 1, 1980·Journal of Immunological Methods·S F de StGroth, D Scheidegger
Sep 1, 1980·The Journal of General Virology·A ZiemieckiK Simons

❮ Previous
Next ❯

Citations

Jul 7, 1995·The Journal of Biological Chemistry·R J StockertA W Wolkoff
Mar 16, 2004·Journal of Virology·Don L GibbonsMargaret Kielian
Sep 19, 2020·Nature Communications·Rebecca S BrownMargaret Kielian
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·P Hilgard, R Stockert
Apr 29, 2016·Journal of Virology·Mathieu DubéMargaret Kielian
May 9, 2000·The Journal of Biological Chemistry·M D Turner, P Arvan
Mar 26, 2010·Viruses·Margaret KielianMaofu Liao
Jul 19, 2013·Journal of Virology·Onyinyechukwu UchimeMargaret Kielian
Dec 5, 2014·PLoS Pathogens·Mathieu DubéMargaret Kielian
Mar 26, 2003·The Journal of General Virology·John A G BriggsStephen D Fuller
Dec 21, 2002·The Journal of Biological Chemistry·Lena HammarR Holland Cheng
Feb 13, 2016·Journal of Virology·Katie M Stiles, Margaret Kielian
Dec 16, 2016·PLoS Pathogens·Maria Guadalupe Martinez, Margaret Kielian
Jan 27, 2004·Molecular Biology of the Cell·Regina KuliawatPeter Arvan
Dec 25, 2013·PLoS Pathogens·Yaw Shin OoiMargaret Kielian
Jan 9, 1999·Journal of Virology·C Smerdou, P Liljeström
Jun 6, 2000·Advanced Drug Delivery Reviews·P J KlasseM Marsh
Mar 9, 2002·Journal of Virology·Anna AhnMargaret Kielian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.