Biosynthesis of [14C]arachidonic acid from [14C]linoleate in primary cultures of rat Sertoli cells

Lipids
J G Coniglio, J Sharp

Abstract

The conversion of [14C]linoleate to [14C]arachidonate by rat Sertoli cells was established by use of primary cultures. Most of the 14C from [1-14C]linoleate was located in C-3 of the synthesized arachidonate, indicating that the labeled tetraene had originated largely by elongation and desaturation of the intact labeled substrate rather than by mere addition of 14C-acetate generated by bio-oxidation of the radioactive substrate to an already existing 18-carbon precursor. Although a relatively small amount of 14C was present in 18:3 omega 6 and a relatively large amount of 14C was present in 20:2, it was not possible from these data to establish the relative importance of 20:2 in the biosynthesis of arachidonic acid in rat Sertoli cells.

Citations

Jan 1, 1994·Progress in Lipid Research·J G Coniglio
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S HuynhA Nouvelot
Aug 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H OulhajA Nouvelot
Mar 22, 2002·The International Journal of Biochemistry & Cell Biology·Graciela E Hurtado de Catalfo, I Nelva T de Gomez Dumm
Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·G E Hurtado de Catalfo, I N De Gómez Dumm
Sep 27, 2012·Cellular and Molecular Life Sciences : CMLS·Marco G AlvesPedro F Oliveira
May 1, 1993·Trends in Biochemical Sciences·H S Hansen
Jun 6, 2021·Toxicology and Applied Pharmacology·A S VallésN E Furland

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