Biosynthesis of a ganoderic acid in Saccharomyces cerevisiae by expressing a cytochrome P450 gene from Ganoderma lucidum

Biotechnology and Bioengineering
Wen-Fang WangJian-Jiang Zhong

Abstract

Ganoderic acid (GA), a triterpenoid from the traditional Chinese medicinal higher fungus Ganoderma lucidum, possesses antitumor and other significant pharmacological activities. Owing to the notorious difficulty and immaturity in genetic manipulation of higher fungi as well as their slow growth, biosynthesis of GAs in a heterologous host is an attractive alternative for their efficient bioproduction. In this study, using Saccharomyces cerevisiae as a host, we did a systematic screening of cytochrome P450 monooxygenase (CYP450) gene candidates from G. lucidum, which may be responsible for the GA biosynthesis from lanosterol but have not been functionally characterized. As a result, overexpression of a CYP450 gene cyp5150l8 was firstly found to produce an antitumor GA, 3-hydroxy-lanosta-8, 24-dien-26 oic acid (HLDOA) in S. cerevisiae, as confirmed by HPLC, LC-MS and NMR. A final titer of 14.5 mg/L of HLDOA was obtained at 120 hr of the yeast fermentation. Furthermore, our in vitro enzymatic experiments indicate that CYP5150L8 catalyzes a three-step biotransformation of lanosterol at C-26 to synthesize HLDOA. To our knowledge, this is the first report on the heterologous biosynthesis of GAs. The results will be helpful to the GA b...Continue Reading

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Citations

Jan 5, 2019·Organic & Biomolecular Chemistry·Hsiao-Ching LinYit-Heng Chooi
Dec 23, 2019·Applied Microbiology and Biotechnology·Ping-An WangJian-Jiang Zhong
Nov 19, 2020·Biotechnology and Bioengineering·Lihong JiangJiazhang Lian
Mar 13, 2021·Natural Product Reports·Xingwang ZhangShengying Li
Aug 31, 2021·Applied Microbiology and Biotechnology·Nico D FessnerAnton Glieder
Dec 19, 2021·Applied Microbiology and Biotechnology·Wen-Fang WangJian-Jiang Zhong

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