PMID: 4881973Nov 1, 1968Paper

Biosynthesis of an insulin precursor by islet tissue of cod (Gadus callarias)

The Biochemical Journal
P T Grant, K B Reid

Abstract

1. At 15 degrees , slices of cod islet tissue incorporated [U-(14)C]proline into proteins soluble in acid-ethanol at a linear rate for 6hr. 2. Initially, all the radioactivity was associated with a polypeptide that had a molecular weight of about 10000 and was appreciably more basic than cod insulin. After 1hr. there was also a significant and progressive increase in the radioactivity of insulin and of fractions intermediate in molecular size and basicity between the polypeptide and insulin. 3. O-Ethyl O-p-nitrophenyl phenylpropylphosphonate markedly decreased the radioactivity both of the intermediate fractions and of insulin, but had no significant effect on the biosynthesis of the polypeptide. In contrast, puromycin inhibited the incorporation of radioactivity into all the fractions. 4. The polypeptide had an activity of less than 0.2 international unit/mg. in the epididymal-fat-pad bioassay. Treatment with low concentrations of trypsin caused a progressive increase in the formation of an insulin-like material, judged by bioassay and ion-exchange chromatography of the digest. 5. Gel filtration of the polypeptide after oxidative sulphitolysis indicated that it was a single polypeptide chain. 6. The results suggest that the po...Continue Reading

Citations

Jul 1, 1980·Clinics in Endocrinology and Metabolism·N Yanaihara
Jul 10, 2007·General and Comparative Endocrinology·Olga HrytsenkoBill Pohajdak
Jun 1, 1978·General and Comparative Endocrinology·D J FletcherE L Hunt
Jun 1, 1978·General and Comparative Endocrinology·D J FletcherE L Hunt
Feb 1, 1982·General and Comparative Endocrinology·A Huth, T A Rapoport
Nov 1, 1972·Clinics in Endocrinology and Metabolism·N R Lazarus
Feb 1, 1972·CRC Critical Reviews in Biochemistry·A E KitabchiS Yu
Aug 1, 1971·Australian and New Zealand Journal of Medicine·J Vallance-Owen
Oct 10, 1977·Biochemical and Biophysical Research Communications·M A Permutt, A Routman
Oct 5, 1970·FEBS Letters·G E. Morris, A Korner
Feb 7, 1970·Nature·A C Trakatellis, G P Schwartz
Jan 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G E Bauer, B D Noe
Jan 1, 1972·Acta diabetologica latina·F Melani
May 15, 1969·The New England Journal of Medicine·D F Steiner
Aug 8, 1974·Biochimica Et Biophysica Acta·A LukowskyT A Rapoport
Jun 1, 1970·Biochimica Et Biophysica Acta·G E Morris, A Korner

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