PMID: 3769927Oct 15, 1986Paper

Biosynthesis of antifreeze polypeptides in the winter flounder. Characterization and seasonal occurrence of precursor polypeptides

European Journal of Biochemistry
C L HewG L Fletcher

Abstract

The precursor proteins for winter flounder antifreeze polypeptide (AFP) were isolated from liver using gel filtration chromatography and reverse-phase high-performance liquid chromatography. Two major pro-antifreezes (Mr 5000), corresponding to the precursors for AFP-6 and AFP-8, were characterized by amino acid analyses and automated Edman degradation. These precursors showed significant antifreeze activity. The pro-antifreezes were synthesized in the liver seasonally as demonstrated by immunoblotting and in vitro liver incorporation studies. No mature AFP were detected in liver, thus indicating that the processing of pro-antifreezes, including amidation of the C-termini, occurred mainly in the serum. The function(s) of the prosequences, if any, remain unclear.

References

Jan 1, 1976·Comparative Biochemistry and Physiology. A, Comparative Physiology·G L KooymanH T Hammel
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G Suchanek, G Kreil
Dec 20, 1977·Biochimica Et Biophysica Acta·A L Devries, Y Lin
Nov 14, 1978·Biochemical and Biophysical Research Communications·C L HewG L Fletcher
Aug 15, 1985·European Journal of Biochemistry·C L HewV S Ananthanarayanan
Jan 15, 1974·Biochemistry·P Y Chou, G D Fasman
Nov 1, 1984·European Journal of Pediatrics·M BeckerO Leiss
Aug 15, 1984·European Journal of Biochemistry·M PickettC Hew
Jan 20, 1983·Biochimica Et Biophysica Acta·M H PickettP L Davies
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·O J YooK L Agarwal
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P L DaviesC L Hew

❮ Previous
Next ❯

Citations

Jun 1, 1996·Protein Science : a Publication of the Protein Society·H ChaoP L Davies
Jun 1, 1989·Fish Physiology and Biochemistry·G L FletcherC L Hew
Oct 1, 1993·Plant Molecular Biology·K D KenwardP L Davies
Mar 1, 1989·Fish Physiology and Biochemistry·G L FletcherM A Shears
Mar 15, 1992·Gene·P L Davies, S Y Gauthier
Jan 1, 1995·Biotechnology Advances·M Griffith, K V Ewart
Sep 15, 2010·The Journal of Biological Chemistry·Peter W WilsonAnthony D J Haymet
May 10, 2002·Bioscience, Biotechnology, and Biochemistry·Yasuhiro YamashitaHitoshi Obata
May 2, 2003·Bioscience, Biotechnology, and Biochemistry·Yasuhiro YamashitaHitoshi Obata
Mar 2, 2018·Extremophiles : Life Under Extreme Conditions·Fernanda P CidMilko A Jorquera
May 14, 2004·Nature·Christopher B MarshallPeter L Davies
Sep 14, 2004·Médecine sciences : M/S·Christopher B MarshallPeter L Davies

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
G K ScottP L Davies
Proceedings of the National Academy of Sciences of the United States of America
P L DaviesC L Hew
© 2021 Meta ULC. All rights reserved