PMID: 9546668Apr 18, 1998Paper

Biosynthesis of C18 polyunsaturated fatty acids in microsomal membrane preparations from the filamentous fungus Mucor circinelloides

European Journal of Biochemistry
F M JacksonG Griffiths

Abstract

The biosynthesis of C18 polyunsaturated fatty acids has been studied in the fungus Mucor circinelloides. Microsomal membrane preparations contained delta9, delta12 and delta6 desaturase activities. The delta9 desaturase exhibited characteristics similar to those of the animal and yeast delta9 desaturases in being membrane bound and utilising stearoyl-CoA as substrate. Cytochrome b5 (a soluble form lacking the 20-amino-acid hydrophobic C-terminus) stimulated desaturation and was identified as a major cytochrome component of the membranes. A high ferricyanide reductase activity (indicative of NADH:cytochrome b5 reductase activity) coupled to inhibition by cyanide further supported the similarity with the mammalian and yeast enzymes. Time-course studies with radiolabelled oleoyl-CoA showed that the oleate [18:1(9)] was transferred to position sn-2 of phosphatidylcholine (PtdCho) and was desaturated to linoleoyl-PtdCho. Removal of the excess oleoyl-CoA from the membranes prior to addition of reductant confirmed that oleoyl-PtdCho is a substrate for the delta12 desaturase. The entry of oleate at this position of the phospholipid was facilitated by the activity of lyso-PtdCho:acyl-CoA acyltransferase (LPCAT), which readily transferre...Continue Reading

Citations

Nov 13, 2004·Applied Microbiology and Biotechnology·Eiji SakuradaniSakayu Shimizu
Oct 20, 2005·Journal of Bioscience and Bioengineering·M Certik, S Shimizu
Jun 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Howard G DamudeNarendra S Yadav
Dec 27, 2011·The Journal of Biological Chemistry·Richard HarkewiczKang Zhang
Dec 9, 2008·Bioscience, Biotechnology, and Biochemistry·Takahiro Oura, Susumu Kajiwara
Aug 1, 2014·Applied Biochemistry and Biotechnology·Cristiano E R ReisBo Hu
Oct 12, 2010·New Biotechnology·Olga P Yurchenko, Randall J Weselake
Nov 25, 2014·Applied Microbiology and Biotechnology·Noemi Ruiz-LopezRichard P Haslam
Apr 2, 2019·Critical Reviews in Biotechnology·Cristiano E Rodrigues ReisHeizir F De Castro
Jul 21, 1998·The Journal of Biological Chemistry·L V MichaelsonA K Stobart
Oct 23, 1998·Microbiology·Frances M JacksonGareth Griffiths
Sep 16, 1999·Biochemical and Biophysical Research Communications·S PassornS Cheevadhanarak

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