Biosynthesis of C9-aldehydes in the moss Physcomitrella patens

Biochimica Et Biophysica Acta
Michael StumpeI Feussner

Abstract

After wounding, the moss Physcomitrella patens emits fatty acid derived volatiles like octenal, octenols and (2E)-nonenal. Flowering plants produce nonenal from C18-fatty acids via lipoxygenase and hydroperoxide lyase reactions, but the moss exploits the C20 precursor arachidonic acid for the formation of these oxylipins. We describe the isolation of the first cDNA (PpHPL) encoding a hydroperoxide lyase from a lower eukaryotic organism. The physiological pathway allocation and characterization of a downstream enal-isomerase gives a new picture for the formation of fatty acid derived volatiles from lower plants. Expression of a fusion protein with a yellow fluorescent protein in moss protoplasts showed that PpHPL was found in clusters in membranes of plastids. PpHPL can be classified as an unspecific hydroperoxide lyase having a substrate preference for 9-hydroperoxides of C18-fatty acids but also the predominant substrate 12-hydroperoxy arachidonic acid is accepted. Feeding experiments using arachidonic acid show an increase in the 12-hydroperoxide being metabolized to C8-aldehydes/alcohols and (3Z)-nonenal, which is rapidly isomerized to (2E)-nonenal. PpHPL knock out lines failed to emit (2E)-nonenal while formation of C8-vola...Continue Reading

Citations

Jun 25, 2009·Planta·Juan Pablo OliverInés Ponce de León
Dec 1, 2012·BMC Plant Biology·Julia ScholzIvo Feussner
Jul 2, 2014·Journal of Molecular Graphics & Modelling·Tuanjai SomboonSeiji Mori
Jan 19, 2010·Phytochemistry·Emmanuel CroisierGeorg Pohnert
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Jul 11, 2021·Plant Molecular Biology·Ambre Guillory, Sandrine Bonhomme
Oct 9, 2019·Journal of Agricultural and Food Chemistry·Holly StolterfohtHarald Pichler
Nov 16, 2021·Frontiers in Plant Science·Moena TanakaKenji Matsui

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