PMID: 2492830Feb 24, 1989Paper

Biosynthesis of collagen crosslinks. II. In vivo labelling and stability of lung collagen in rats

Biochimica Et Biophysica Acta
J A LastK Reiser

Abstract

Rat lung collagen was labelled in vivo by a single intraperitoneal injection of [3H]lysine at several key timepoints in lung development: days 11 (alveolar proliferation), 26 (start of equilibrated growth), 42 (end of equilibrated growth), and 100 (adult lung structure present). The rates of deposition of labelled hydroxylysine and the difunctional, Schiff base-derived crosslinks hydroxylysinonorleucine (HLNL) and dihydroxylysinonorleucine (DHLNL) were quantified. We also measured total lung content of the trifunctional, mature crosslink hydroxypyridinium (OHP) in these same animals. While the relative rates of accumulation of labelled collagen [3H]hydroxylysine differed by a factor of about 6 at the different times of injection of labelled precursor, quantitative and qualitative patterns of collagen crosslinking were very similar at all of the lung developmental stages studied. Furthermore, there was little or no breakdown of the lung collagen pool as defined by the presence of labelled crosslinks; changes in lung DHLNL content could be completely accounted for by its maturation to OHP, regardless of the age of the rats when injected with the radioactive precursor. We conclude that mature, crosslinked collagen in the lungs of ...Continue Reading

References

Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R NissenS Udenfriend
Feb 13, 1975·Biochimica Et Biophysica Acta·S H Jackson, J A Heininger
Mar 1, 1986·Journal of Biochemical Toxicology·K M ReiserH R Witschi
Mar 15, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·S H Jackson, J A Heininger
Jan 1, 1967·The Biochemical Journal·M E NimniL A Bavetta
Oct 1, 1984·Collagen and Related Research·R S Bienkowski
Aug 15, 1981·The Biochemical Journal·J N HildebranR B Low
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R A BergR G Crystal
Jul 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·E A Nardell, J S Brody
Feb 5, 1981·Biochimica Et Biophysica Acta·M A DubickJ A Last
Jan 1, 1961·Proceedings of the Society for Experimental Biology and Medicine·K Y KAOT H MCGAVACK
Nov 1, 1964·Proceedings of the Society for Experimental Biology and Medicine·M E NIMNI, L A BAVETTA

❮ Previous
Next ❯

Citations

Jan 1, 1990·The International Journal of Biochemistry·J A LastK M Reiser
Nov 5, 1997·British Poultry Science·J L AriasE Kessi
Sep 24, 2011·American Journal of Respiratory Cell and Molecular Biology·Michael M BundesmannLynn M Schnapp
Jan 1, 1996·Connective Tissue Research·S G VellemanR J McCormick
Jan 1, 1994·Drug Metabolism Reviews·P L SkipperS R Tannenbaum
Jan 18, 2018·Avian Diseases·Sandra G VellemanJeffrey R Tonniges
Apr 9, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·William McKleroyKamran Atabai
Jun 8, 2021·Frontiers in Medicine·Ceylan OnursalClaudia A Staab-Weijnitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.