Biosynthesis of collagen crosslinks. III. In vivo labeling and stability of lung collagen in rats with bleomycin-induced pulmonary fibrosis.

American Journal of Respiratory Cell and Molecular Biology
J A Last, K M Reiser

Abstract

Rats were injected intraperitoneally with 1 mCi (each) of [3H]lysine at Day 11 of neonatal life to label their lung collagen. Five weeks later, half of the animals were given an intratracheal injection of 1.5 U of bleomycin sulfate via a tracheostomy; control animals received saline intratracheally by the same technique. Age-matched groups of control and bleomycin-treated rats were killed, and their lung collagen was analyzed at zero (control animals only), 1, 2, 4, 6, and 10 wk after bleomycin administration, a time course appropriate for development of pulmonary fibrosis in this animal model. We measured radioactivity in hydroxylysine and in the difunctional collagen crosslinks hydroxylysinonorleucine and dihydroxylysinonorleucine at each time point. No evidence of breakdown of this pool of mature, preformed collagen was observed in lungs of either the control or the bleomycin-treated rats. We also measured the total lung content of hydroxypyridinium, a trifunctional collagen crosslink, by its intrinsic fluorescence. There was no evidence of collagen degradation in lungs of either group of rats by this criterion either. We conclude that there is no biochemically detectable turnover of mature lung collagen, defined as that poo...Continue Reading

References

Oct 4, 1979·The New England Journal of Medicine·J E GadekR G Crystal
Apr 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L Klein, J ChandraRajan
Mar 1, 1986·Journal of Biochemical Toxicology·K M ReiserH R Witschi
Jul 1, 1987·Toxicology and Applied Pharmacology·K M ReiserJ A Last
Oct 1, 1986·Collagen and Related Research·K M Reiser, J A Last
Sep 15, 1981·Toxicology and Applied Pharmacology·T W HesterbergJ A Last
Oct 1, 1984·Collagen and Related Research·R S Bienkowski
Jan 1, 1983·The American Review of Respiratory Disease·W M HaschekH P Witschi
Jul 1, 1981·The Journal of Clinical Investigation·G W HunninghakeR G Crystal

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J A LastK M Reiser
Mar 18, 2003·Toxicology and Applied Pharmacology·Nicholas J KenyonJerold A Last
Aug 19, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Terence E McIffAgostino Molteni
Oct 1, 1990·Zentralblatt für Veterinärmedizin. Reihe A·N C Winder, R von Fellenberg
Jul 1, 1997·British Journal of Pharmacology·J SchloosC Bruns
Jun 30, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M HoshinoS Isogai
Jun 8, 2021·Frontiers in Medicine·Ceylan OnursalClaudia A Staab-Weijnitz

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