Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus.

Journal of Biotechnology
Jennifer Hage-HülsmannThomas Drepper

Abstract

Cyclic triterpenes are a large group of secondary metabolites produced by plants, fungi and bacteria. They have diverse biological functions, and offer potential health benefits for humans. Although various terpenes from the mono-, sesqui- and diterpene classes are easy to produce in engineered bacteria, heterologous synthesis of cyclic triterpenes is more challenging. We have recently shown that the triterpene cycloartenol can be produced in Rhodobacter capsulatus SB1003 but initial titers were low with 0.34mgL-1. To assess, if this phototrophic α-proteobacterium can be engineered for enhanced triterpene production, we followed two alternative strategies by comparing the performance of the R. capsulatus SB1003 wildtype strain with two recombinant strains carrying either a mevalonate pathway implemented from Paracoccus zeaxanthinifaciens or a deletion in the intrinsic carotenoid biosynthesis gene crtE. These strains are thus engineered for an enhanced isoprenoid biosynthesis or a suppressed precursor conversion by the competing carotenoid pathway. Moreover, three different cycloartenol synthase (CAS) genes from Arabidopsis thaliana or the myxobacterial strains Stigmatella aurantiaca Sga15 and DW4/3-1 were tested for heterologou...Continue Reading

References

Sep 15, 1981·Biochimica Et Biophysica Acta·G F Barnard, G Popják
Jul 1, 1993·Archives of Biochemistry and Biophysics·D ZhangC D Poulter
Jan 1, 1997·Annual Review of Microbiology·G A Armstrong
Jan 24, 2007·Metabolic Engineering·Douglas J PiteraJay D Keasling
Oct 22, 2013·Nature Biotechnology·Robert H DahlJay D Keasling
Feb 7, 2014·Annual Review of Plant Biology·Ramesha ThimmappaAnne Osbourn
Mar 3, 2015·Biotechnology and Bioengineering·Nymul E KhanWayne R Curtis
Jul 19, 2016·Journal of Applied Microbiology·Y Li, G Wang
Jul 26, 2016·Frontiers in Microbiology·Jeremy H WeiPaula V Welander
Dec 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Amy B BantaPaula V Welander
Jan 18, 2017·Nature Communications·Jaeok ParkAlbert M Berghuis
Apr 19, 2017·Journal of Agricultural and Food Chemistry·Florence M SchemppJens Schrader
Nov 16, 2017·International Journal of Molecular Sciences·Mei Lan JinOk Tae Kim
Aug 17, 2018·Current Opinion in Biotechnology·Nicolai KallscheuerJan Marienhagen
Feb 17, 2019·Molecular Biology Reports·Ahmed Adebayo Ishola, Kayode Ezekiel Adewole
May 28, 2019·Applied Microbiology and Biotechnology·Sandra Moser, Harald Pichler
Jun 13, 2019·Journal of Industrial Microbiology & Biotechnology·Enrico OrsiRuud A Weusthuis

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.