PMID: 16657380Jun 1, 1970Paper

Biosynthesis of cytidine diphosphate diglyceride by enzyme preparations from cauliflower

Plant Physiology
S Sumida, J B Mudd


The activity of the enzyme responsible for the synthesis of cytidine diphosphate diglyceride has been found in the 20,000g pellet of a cauliflower (Brassica oleracea) inflorescence homogenate. Greater enzyme activity was found in the pellet after treatment with 0.02% Triton X-100, or after freezing and thawing. Activity was greater when fluoride was included in the reaction mixture. The enzyme has a pH optimum of 5.6, an optimal Mn(2+) concentration of 1mm and an optimal Mg(2+) concentration of 30 mm. At the optimal concentration, Mn(2+) was 4 times better than Mg(2+). The utilization of biosynthesized cytidine diphosphate diglyceride can be enhanced by addition of inositol, thereby causing the formation of phosphatidyl inositol.


Dec 18, 1968·Biochimica Et Biophysica Acta·H T Hutchison, J E Cronan
Sep 1, 1954·Canadian Journal of Biochemistry and Physiology·M KATES
Jul 1, 1955·Canadian Journal of Biochemistry and Physiology·M KATES
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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