Biosynthesis of Fe3 O4 @Ag Nanocomposite and Evaluation of Its Performance on Expression of norA and norB Efflux Pump Genes in Ciprofloxacin-Resistant Staphylococcus aureus

Biological Trace Element Research
Nastaran ShokoofehAli Salehzadeh

Abstract

At present, the universal health problem with Staphylococcus aureus is the emergence of multidrug-resistant strains due to the overuse of antibiotics. Drug extrusion through efflux pumps is one of the bacterial mechanisms to neutralize the bactericidal effect of antibiotics. The antibacterial activity of silver nanoparticle as well as Fe3O4 nanoparticle had been previously studied and widely described. Today, the development of green methods for nanomaterial synthesis is an important aspect of research in the field of nanotechnology. Here, we report the biosynthesis and characterization of Fe3O4@Ag nanocomposite by Spirulina platensis cyanobacterium and it impacts on the expression of efflux pump genes in ciprofloxacin-resistant S. aureus (CRSA). The physical properties of biosynthesized nanocomposite measured and confirmed by ultraviolet-visible spectroscopy, Fourier-transform infrared spectroscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and scanning and transmission electron microscopy. The minimum inhibitory concentration (MIC) of ciprofloxacin in CRSA strains was determined in the presence of Fe3O4@Ag nanoparticles by broth microdilution method. The effect of Fe3O4@Ag nanocomposite on the expression of nor...Continue Reading

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Citations

May 8, 2020·Biological Trace Element Research·Salem S Salem, Amr Fouda
Nov 18, 2020·Biological Trace Element Research·Nermeen EldebanyHoda Elkhenany
Oct 30, 2020·International Journal of Nanomedicine·Chih-Chien HuSteve Wen-Neng Ueng

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Methods Mentioned

BETA
scanning electron microscopy
X-ray
PCR
surface plasmon resonance
electron microscopy

Software Mentioned

Excel
SPSS

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