PMID: 23841Feb 10, 1978

Biosynthesis of glycogen in Neurospora crassa. Purification and properties of the UDPglucose:glycogen 4-alpha-glucosyltransferase

Biochimica Et Biophysica Acta
H Takahara, K Matsuda

Abstract

The Neurospora crassa glycogen synthase (UDPglucose:glycogen 4-alpha-glucosyltransferase, EC 2.4.1.11) was purified to electrophoretic homogeneity by a procedure involving ultracentrifugation, DEAE-cellulose column chromatography, (NH4)2SO4 fractionation and 3-aminopropyl-Sepharose column chromatography. The final purified enzyme preparation was almost entirely dependent on glucose-6-P and had a specific activity of 6.9 units per mg of protein. The subunit molecular weight of the glycogen synthase was determined by electrophoresis in sodium dodecyl sulfate-polyacrylamide gel to be 88 000--90 000. The native enzyme was shown to have a molecular weight of 270 000 as determined by sucrose density gradient centrifugation. Thus, the glucose-6-P-dependent form of the N. crassa glycogen synthase can exist as trimer of the subunit. Limited proteolysis with trypsin or chymotrypsin converted the glucose-6-P-dependent form of the enzyme into an apparent glucose-6-P-independent form. The enzyme was shown to catalyze transfer of glucose from UDPglucose to glycogen as well as to its phosphorylase limit dextrin, but not to its beta-amylase limit dextrin. Moreover, glucose, maltose and maltotriose were not active as acceptors.

References

Mar 28, 1986·Biochemical and Biophysical Research Communications·W M RutherfordM J Nadakavukaren
Mar 13, 2016·The Journal of Biological Chemistry·Sandy RaynaudÁngel Mérida

Citations

Mar 23, 1976·Biochemistry·S D Killilea, W J Whelan
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·S Shaltiel, Z Er-El
Aug 13, 1974·Biochemistry·P H McVerry, K H Kim
Nov 21, 1972·Biochemistry·D C LinE J Massaro
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·L B Rothman-Denes, E Cabib
Aug 1, 1969·Biochemistry·L B Rothman, E Cabib

Related Concepts

Neurospora crassa
Macromolecular Compounds
Centrifugation, Density Gradient
Cations, Divalent
Neurospora
Polyacrylamide Gels
Dextrin
Peptide Hydrolases
Glycogen Storage Disease Type I
Trypsin

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