PMID: 6403553Mar 1, 1983Paper

Biosynthesis of high density lipoprotein by chicken liver: nature of nascent intracellular high density lipoprotein

The Journal of Cell Biology
D Banerjee, C M Redman

Abstract

Young chickens were administered L-[(3)H]leucine and after 10 or 30 min the livers were removed and fractioned into rough (RER) and smooth (SER) endoplasmic reticulum fractions and into light, intermediate, and heavy golgo cell fractions. The labeled high density lipoprotein (HDL), contained within these intracellular organelles was isolated either by immunoprecipitation using rabbit antiserum to rooster HDL, or by ultracentrifugal glotation between densities 1.063 and 1.21 g/ml. The radioactive apoproteins of nascent HDL were analyzed by SDS PAGE and detected by fluorography. Analyses of radioactive apoproteins obtained by immunoprecipitation from the contents of the RER, the SER, and the three golgi complex fractions revealed only one apoprotein, A1. The C peptide present in serum HDL was not detected intracellularly. The radioactive apoprotein A1 which is present within the cisternae of the RER and the SER fractions failed to float, whereas apoprotein A1, present within the golgi apparatus, readily floated between densities 1.063 and 1.21 g/ml. The HDL particles, isolated by flotation from the golgi apparatus content, were further characterized by lipid and protein analyses and by electron microscopy. Golgi HDL particles hav...Continue Reading

References

Oct 21, 1975·Biochimica Et Biophysica Acta·A W Kruski, A M Scanu
Feb 20, 1976·Biochimica Et Biophysica Acta·R L JacksonA R Means
May 1, 1976·The Journal of Cell Biology·C A AlexanderR J Havel
Jul 1, 1976·Canadian Journal of Biochemistry·A C Nestruck, D Rubinstein
Sep 1, 1976·The Journal of Clinical Investigation·R L HamiltonR J Havel
Jan 1, 1978·Annual Review of Biochemistry·L C SmithA M Gotto
Feb 26, 1979·Biochimica Et Biophysica Acta·D J KudzmaE N Ellis
Feb 1, 1975·The Journal of Cell Biology·H GlaumannJ L Ericsson
Jul 1, 1975·The Journal of Cell Biology·C M RedmanG E Palade
Aug 15, 1975·European Journal of Biochemistry·R A Laskey, A D Mills
Feb 15, 1972·Biochemistry·L A HillyardS A Pangburn
Oct 1, 1973·The Journal of Cell Biology·J J BergeronG E Palade
Dec 20, 1973·Biochimica Et Biophysica Acta·S EisenbergF T Lindgren
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Mar 1, 1982·The Journal of Cell Biology·K E Howell, G E Palade
Mar 1, 1982·The Journal of Cell Biology·K E Howell, G E Palade
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON

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Citations

Feb 18, 1999·Journal of Cellular Biochemistry·L VleurickP P Van Veldhoven
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P C Allen
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y M LiH Vlassara
Jan 15, 2005·Current Opinion in Lipidology·Ji-Young Lee, John S Parks
Nov 19, 2005·Expert Review of Cardiovascular Therapy·Jerzy-Roch NoferGerd Assmann
Nov 1, 1988·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J Havel, R L Hamilton
Jan 1, 1984·Journal of Cellular Biochemistry·D R JaneroM D Lane
Mar 31, 2005·The Journal of Biological Chemistry·Hui ZhengYves L Marcel
Jan 28, 2003·The Journal of Biological Chemistry·Robert S KissYves L Marcel
Oct 6, 2005·The Journal of Biological Chemistry·Jovana MaricYves L Marcel
Feb 19, 2000·Biochemical and Biophysical Research Communications·H JaegerF Berr
Sep 21, 1989·Biochimica Et Biophysica Acta·J L DixonD Banerjee

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