PMID: 5257135Jun 1, 1969Paper

Biosynthesis of insulin in bovine fetal pancreatic slices: the incorporation of tritiated leucine into a single-chain proinsulin, a double-chain intermediate, and insulin in subcellular fractions

Proceedings of the National Academy of Sciences of the United States of America
A K Tung, C C Yip

Abstract

This communication reports the biosynthesis of insulin in the bovine fetal pancreatic slices in vitro. Double-chain proinsulin and insulin were found as major components in the mitochondrial-granule fraction of bovine fetal pancreas. Tritiated leucine was incorporated into a single-chain proinsulin, a double-chain proinsulin, and insulin. Subcellular fractionation of the slices incubated with tritiated leucine showed that radioactive single-chain proinsulin was present in the deoxycholate-soluble microsomal fraction, deoxycholate-insoluble microsomal fraction, and mitochondrial-granule fraction. Labeled double-chain proinsulin and insulin were present in the deoxycholate-soluble microsomal and mitochondrial-granule fractions. These results are consistent with the hypothesis that insulin is synthesized as a single-chain polypeptide on the ribosomes, and that intracellular proteolysis in the subcellular membranous organelles and beta-granules converts the single-chain proinsulin to insulin via a double-chain intermediate.

References

Jun 22, 1968·Lancet·A H RubensteinD F Steiner
Feb 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·C C Yip, J Logothetopoulos
Nov 17, 1967·Biochemical and Biophysical Research Communications·C C Yip, B J Lin
Jul 12, 1968·Science·R E ChanceW W Bromer
Jul 1, 1960·The Journal of Clinical Investigation·G M GRODSKY, P H FORSHAM

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Citations

Oct 1, 1974·Biulleten' eksperimental'noĭ biologii i meditsiny·S A Morenkova
Jan 1, 1980·Pharmacology & Therapeutics·G Telegdy
Jun 7, 1979·The New England Journal of Medicine·O K ole-MoiYoiK F Austen
Jun 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·C C Yip
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C C YipH Hsu
Aug 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·O O YoiK F Austen
Jul 1, 1970·The Journal of Clinical Investigation·T TaneseL Recant
Jan 28, 1971·Biochimica Et Biophysica Acta·J Sy, K S McCarty
Oct 15, 1971·Biochemical and Biophysical Research Communications·A K Tung, F Zerega
Feb 1, 1972·CRC Critical Reviews in Biochemistry·A E KitabchiS Yu

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