PMID: 6407473Feb 15, 1983Paper

Biosynthesis of intestinal microvillar proteins. Pulse-chase labelling studies on maltase-glucoamylase, aminopeptidase A and dipeptidyl peptidase IV

The Biochemical Journal
E M DanielsenO Norén

Abstract

The biogenesis of three intestinal microvillar enzymes, maltase-glucoamylase (EC 3.2.1.20), aminopeptidase A (aspartate aminopeptidase, EC 3.4.11.7) and dipeptidyl peptidase IV (EC 3.4.14.5), was studied by pulse-chase labelling of pig small-intestinal explants kept in organ culture. The earliest detectable forms of the enzymes were polypeptides of Mr 225000, 140000 and 115000 respectively. These were found to represent the enzymes in a 'high-mannose' state of glycosylation, as judged by their susceptibility to treatment with endo-beta-N-acetylglucosaminidase H (EC 3.2.1.96). After about 40-60 min of chase, maltase-glucoamylase, aminopeptidase A and dipeptidyl peptidase IV were further modified to yield the mature polypeptides of Mr 245000, 170000 and 137000 respectively, which were expressed at the microvillar membrane after 60-90 min of chase. The fact that the enzymes before reaching the microvillar membrane were found in a Ca2+-precipitated membrane fraction (intracellular and basolateral membranes), but not in soluble form, indicates that during biogenesis maltase-glucoamylase, aminopeptidase A and dipeptidyl peptidase IV are transported and assembled in a membrane-bound state.

Citations

Aug 2, 2002·Biochemical and Biophysical Research Communications·Roberto Quezada-CalvilloBrian J Underdown
Feb 28, 1998·The Journal of Biological Chemistry·B L NicholsE Sterchi
Jan 1, 1995·Critical Reviews in Biochemistry and Molecular Biology·E H Van BeersJ Dekker
May 16, 2018·Journal of Pediatric Gastroenterology and Nutrition·Byung-Hoo Lee, Bruce R Hamaker
Sep 14, 2001·Scandinavian Journal of Immunology·M D GorrellG W McCaughan
Jun 5, 1998·The International Journal of Biochemistry & Cell Biology·V OosthuizenH Kamiya
Aug 1, 1983·Journal of Biochemical and Biophysical Methods·E M Danielsen, G M Cowell
Jul 18, 1984·Biochemical and Biophysical Research Communications·E M DanielsenH Sjöström
Jan 14, 1986·Biochemical and Biophysical Research Communications·D H AlpersA W Strauss

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