PMID: 6540204Jul 9, 1984Paper

Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants

FEBS Letters
G M Cowell, E M Danielsen

Abstract

Using alkaline extraction to separate cytoskeletal and membrane proteins of intestinal microvilli, the kinetics of assembly of these two microvillar protein compartments was studied by pulse-chase labelling of pig small intestinal mucosal explants, kept in organ culture. Following a 10 min pulse of [35S]methionine, the membrane proteins did not appear in the microvillar fraction until after 40-60 min of chase. In contrast, the cytoskeletal components, of which the 110-kDa protein and villin were immunologically identified, were expressed in the microvillar fraction immediately after the 10 min pulse. These different kinetics of appearance indicate that the two microvillar protein compartments have separate mechanisms of biosynthesis and microvillar expression.

References

Dec 1, 1979·The Journal of Cell Biology·P T Matsudaira, D R Burgess
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Oct 1, 1972·The Journal of Clinical Investigation·D H Alpers
Aug 1, 1969·The Journal of Clinical Investigation·T H Browning, J S Trier
Jan 1, 1982·Physiological Reviews·A J Kenny, S Maroux
Nov 1, 1983·Cell·M S Mooseker
Feb 1, 1984·The Journal of Cell Biology·R P StidwillD R Burgess
Sep 1, 1982·European Journal of Biochemistry·S H SørensenE M Danielsen

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Citations

Dec 1, 1989·Gut·R Holmes, R W Lobley
Nov 4, 1985·European Journal of Biochemistry·J W MuldersW W de Jong
Oct 11, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tilman GruneKelvin J A Davies

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