Biosynthesis of kitasamycin (leucomycin) by leucine analog-resistant mutants of Streptomyces kitasatoensis.

Antimicrobial Agents and Chemotherapy
C VézinaP Audet

Abstract

The biosynthesis of kitasamycin in Streptomyces kitasatoensis B-896 was profoundly influenced by the addition of precursors to complex and defined media: l-valine and l-leucine directed biosynthesis towards the pairs A(4)/A(5) (R(2) = butyryl) and A(1)/A(3) (R(2) = isovaleryl), respectively, and total kitasamycin titers were doubled and quadrupled, respectively. S. kitasatoensis B-896 was very resistant (>20 mg/ml) to alpha-aminobutyric acid, an analog of l-valine, but very susceptible to l-leucine analogs 5', 5', 5'-trifluoroleucine and 4-azaleucine (5 to 10 mug/ml). The inhibition by 4-azaleucine could be reversed by l-leucine, but by none of the other amino acids of the pyruvate family or the amino acids of the aspartate pathway. 4-Azaleucine-resistant mutants were isolated which in the absence of any precursors overproduced l-leucine and a kitasamycin complex mainly consisting of the pair A(1)/A(3). These 4-azaleucine-resistant mutants are presumed to be regulatory mutants in which alpha-isopropylmalate synthase, the first enzyme of the l-leucine pathway, has become either derepressed or desensitized to leucine feedback inhibition. l-Leucine-regulatory mutants have economic value: in the absence of expensive precursors, the...Continue Reading

References

Jan 1, 1978·Annual Review of Microbiology·S W QueenerC Vézina
Jan 1, 1978·Annual Review of Biochemistry·H E Umbarger
Feb 1, 1977·The Journal of Antibiotics·S OmuraM Aizawa
Oct 1, 1976·The Journal of Antibiotics·S OmuraK Atsumi
Jun 1, 1975·The Journal of Antibiotics·S Omura, A Nakagawa
Mar 1, 1974·The Journal of Antibiotics·S Rakhit, K Singh
Jan 1, 1973·Advances in Applied Microbiology·A L Demain
Sep 1, 1973·Bacteriological Reviews·D A HopwoodA Vivian
Dec 1, 1973·The Journal of Antibiotics·S OmuraT Hata
Jun 1, 1974·Journal of Bacteriology·B I Stieglitz, J M Calvo
Jan 15, 1963·Experientia·H GRISEBACH, H ACHENBACH

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Citations

Jan 1, 1982·Folia Microbiologica·S PospísilZ Vanĕk
Dec 31, 2008·Bioprocess and Biosystems Engineering·Zhen-Lin LiSi-Liang Zhang
Jan 14, 2011·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Masakazu Horie
Mar 18, 2008·Journal of Pharmaceutical and Biomedical Analysis·F DaidoneE Adams
Mar 5, 2015·Chemical Society Reviews·Sherif I ElshahawiJon S Thorson
Feb 14, 2012·Journal of Pharmaceutical and Biomedical Analysis·Larissa Van den BosscheErwin Adams
Apr 14, 2017·Environmental Science & Technology·Alberto PasamontesCristina E Davis
Jan 1, 1981·Zeitschrift für allgemeine Mikrobiologie·U Gräfe
Sep 5, 2015·Biotechnology and Applied Biochemistry·Qianli Zheng, Shuhong Gao

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