PMID: 737184Dec 21, 1978Paper

Biosynthesis of low molecular weight RNA in mouse myeloma cells

Biochimica Et Biophysica Acta
A Brown, W F Marzluff

Abstract

The low molecular weight RNAs in the nucleus and cytoplasm of mouse myeloma cells have been characterized. There are major nuclear species (other than 5-S and tRNA), four of which contain 'capped' 5' termini. There are three major small cytoplasmic RNAs none of which contain 'caps'. The biosynthesis of the nuclear species when studied using [3H]adenosine as an RNA precursor is characterized by a rapid, transient appearance in the cytoplasm, followed by fixation in the nucleus. Within 15 min, the amount in the cytoplasm has reached a steady-state level maintained for 60 min, while the accumulation in nuclei is linear after a short lag (less than 5 min). When biosynthesis is studied using [Me-3H]methionine as a precursor, much less labeled RNA is present in the cytoplasm, suggesting that methylation may immediately precede fixation into the nucleus.

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Citations

Aug 26, 1980·Biochimica Et Biophysica Acta·P Hellung-LarsenS Frederiksen
Dec 1, 1987·Molecular and Cellular Biology·S M Lobo, W F Marzluff

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