Biosynthesis of Poly(3-Hydroxyalkanoic Acid) Copolymer from CO(inf2) in Pseudomonas acidophila through Introduction of the DNA Fragment Responsible for Chemolithoautotrophic Growth of Alcaligenes hydrogenophilus.

Applied and Environmental Microbiology
K YagiT Mizoguchi

Abstract

Pseudomonas acidophila is a bacterial strain producing a poly(3-hydroxyalkanoic acid) (PHA) copolymer from low-molecular-weight organic compounds such as formate and acetate. The genes responsible for PHA production were cloned in cosmid pIK7 containing a 14.8-kb HindIII fragment of P. acidophila DNA. With the aim of developing a means of producing a PHA copolymer from CO(inf2), cosmid pIK7 was introduced into a polymer-negative mutant of the chemolithoautotrophic bacterium Alcaligenes eutrophus PHB(sup-)4. However, the recombinant strain produced a homopolymer of 3-hydroxybutyric acid (polyhydroxybutyric acid) from CO(inf2). Since it was thought that the composition of the accumulated polymer might depend not on the PHA biosynthetic genes but on the metabolism of the host strain, a recombinant plasmid, pFUS, containing the genes for chemolithoautotrophic growth of the hydrogen-oxidizing bacterium A. hydrogenophilus was introduced into P. acidophila by conjugation. The recombinant plasmid pFUS was stably maintained in P. acidophila in the absence of chemolithoautotrophic or antibiotic selection. This pFUS-harboring strain possessed the ability to grow under a gas mixture of H(inf2), O(inf2), and CO(inf2) in a mineral salts medi...Continue Reading

References

Mar 30, 1976·Molecular & General Genetics : MGG·D Haas, B W Holloway
May 29, 1986·Biochemical and Biophysical Research Communications·K YagiY Miura
Jan 1, 1970·Archiv für Mikrobiologie·H G SchlegelI Krauss
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Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Mar 1, 1965·Journal of Molecular Biology·K I BERNS, C A THOMAS

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Citations

Jun 17, 1998·Applied Biochemistry and Biotechnology·F UmedaT Mizoguchi
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Viviana UrtuviaMichael Seeger
Feb 5, 2019·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kiyohito Yagi

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