Biosynthesis of poly(glycolate-co-lactate-co-3-hydroxybutyrate) from glucose by metabolically engineered Escherichia coli

Metabolic Engineering
Zheng-Jun LiGregory Stephanopoulos

Abstract

Metabolically engineered Escherichia coli strains were constructed to effectively produce novel glycolate-containing biopolymers from glucose. First, the glyoxylate bypass pathway and glyoxylate reductase were engineered such as to generate glycolate. Second, glycolate and lactate were activated by the Megasphaera elsdenii propionyl-CoA transferase to synthesize glycolyl-CoA and lactyl-CoA, respectively. Third, β-ketothiolase and acetoacetyl-CoA reductase from Ralstonia eutropha were introduced to synthesize 3-hydroxybutyryl-CoA from acetyl-CoA. At last, the Ser325Thr/Gln481Lys mutant of polyhydroxyalkanoate (PHA) synthase from Pseudomonas sp. 61-3 was over-expressed to polymerize glycolyl-CoA, lactyl-CoA and 3-hydroxybutyryl-CoA to produce poly(glycolate-co-lactate-co-3-hydroxybutyrate). The recombinant E. coli was able to accumulate the novel terpolymer with a titer of 3.90g/l in shake flask cultures. The structure of the resulting polymer was chemically characterized by proton NMR analysis. Assessment of thermal and mechanical properties demonstrated that the produced terpolymer possessed decreased crystallinity and improved toughness, in comparison to poly(3-hydroxybutyrate) homopolymer. This is the first study reporting ef...Continue Reading

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Citations

Mar 10, 2019·Biotechnology Journal·Youngjoon LeeSang Y Lee
Apr 14, 2020·Biotechnology and Bioengineering·So Young ChoiSang Yup Lee
Oct 24, 2019·Mini Reviews in Medicinal Chemistry·Mengxun ShiMo Xian
Apr 7, 2020·Advanced Materials·So Young ChoiSang Yup Lee
Nov 20, 2016·Microbial Biotechnology·Yilin RenGuo-Qiang Chen
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dattatray K BedadeRichard A Gross
Jul 30, 2021·Advanced Materials·Mengyi LiGuo-Qiang Chen

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