Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase

Biotechnology and Bioengineering
Taek Ho YangSang Yup Lee

Abstract

For the synthesis of polylactic acid (PLA) and its copolymers by one-step fermentation process, heterologous pathways involving Clostridium propionicum propionate CoA transferase (Pct(Cp)) and Pseudomonas sp. MBEL 6-19 polyhydroxyalkanoate (PHA) synthase 1 (PhaC1(Ps6-19)) were introduced into Escherichia coli for the generation of lactyl-CoA endogenously and incorporation of lactyl-CoA into the polymer, respectively. Since the wild-type PhaC1(Ps6-19) did not efficiently accept lactyl-CoA as a substrate, site directed mutagenesis as well as saturation mutagenesis were performed to improve the enzyme. The wild-type Pct(Cp) was not able to efficiently convert lactate to lactyl-CoA and was found to exert inhibitory effect on cell growth, random mutagenesis by error-prone PCR was carried out. By employing engineered PhaC1(Ps6-19) and Pct(Cp), poly(3-hydroxybutyrate-co-lactate), P(3HB-co-LA), containing 20-49 mol% lactate could be produced up to 62 wt% from glucose and 3HB. By controlling the 3HB concentration in the medium, PLA homopolymer and P(3HB-co-LA) containing lactate as a major monomer unit could be synthesized. Also, P(3HB-co-LA) copolymers containing various lactate fractions could be produced from glucose alone by introdu...Continue Reading

References

Sep 5, 2003·The Biochemical Journal·Bernd H A Rehm
Oct 7, 2004·Macromolecular Bioscience·Isao NodaCurtis Marcott
Jun 11, 2005·Biomaterials·Guo-Qiang Chen, Qiong Wu
Mar 21, 2007·Biomacromolecules·Dahlia HaynesDennis W Smith
Jan 5, 1996·Biotechnology and Bioengineering·S Y Lee
Nov 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Seiichi TaguchiShusei Obata
Nov 26, 2009·Biotechnology and Bioengineering·Yu Kyung JungSang Yup Lee

❮ Previous
Next ❯

Citations

Dec 3, 2010·Applied Microbiology and Biotechnology·Danilo PorroDiethard Mattanovich
Nov 29, 2012·Applied Microbiology and Biotechnology·Chuan XuMing Chen
Mar 12, 2013·Applied Microbiology and Biotechnology·Qian WangQingsheng Qi
Jun 7, 2013·Applied Biochemistry and Biotechnology·Jae Hyung LeeKi Jun Jeong
Jun 29, 2010·Nature Reviews. Microbiology·Bernd H A Rehm
Apr 2, 2013·Annual Review of Chemical and Biomolecular Engineering·Benjamin M WoolstonGregory Stephanopoulos
May 30, 2013·Microbial Cell Factories·Gi Na Lee, Jonguk Na
Sep 24, 2013·Applied Microbiology and Biotechnology·Elena VolodinaAlexander Steinbüchel
Jan 3, 2013·Biotechnology Advances·Jae Ho ShinSang Yup Lee
Apr 30, 2013·Biotechnology Advances·Mohamed Ali Abdel-RahmanKenji Sonomoto
May 27, 2014·Applied Microbiology and Biotechnology·Björn AndreeßenAlexander Steinbüchel
May 19, 2012·Nature Chemical Biology·Jeong Wook LeeSang Yup Lee
Dec 8, 2015·Canadian Journal of Microbiology·Tam T Tran, Trevor C Charles
Jan 15, 2015·Current Opinion in Biotechnology·Konrad B Otte, Bernhard Hauer
Oct 12, 2015·Applied Microbiology and Biotechnology·Lingfeng ZhuYanhe Ma
Jul 7, 2011·Journal of Biotechnology·Mohamed Ali Abdel-RahmanKenji Sonomoto
May 24, 2011·Current Opinion in Structural Biology·Himanshu Dhamankar, Kristala L J Prather
May 13, 2011·Trends in Biotechnology·Jeong Wook LeeSang Yup Lee
Mar 12, 2010·Current Opinion in Microbiology·Dokyun NaSang Yup Lee
Nov 8, 2011·Biotechnology Journal·Si Jae ParkTaek Ho Yang
Jul 29, 2010·Biotechnology Journal·Nathaniel D MaynardMarkus W Covert
Apr 17, 2013·Biotechnology Journal·Zhen Chen, An-Ping Zeng
Jul 7, 2012·Biotechnology and Bioengineering·Yu-Sin JangSang Yup Lee
Nov 26, 2009·Biotechnology and Bioengineering·Yu Kyung JungSang Yup Lee
Mar 31, 2010·EMBO Reports·Philip Hunter
Sep 27, 2014·Biotechnology and Bioengineering·Si Jae ParkSang Yup Lee
Apr 15, 2015·Biotechnology Advances·Hao QiYing-Jin Yuan
Jul 3, 2015·Applied Microbiology and Biotechnology·Takeharu TsugeKouhei Mizuno
Mar 23, 2011·Current Opinion in Biotechnology·Jeong Wook LeeSang Yup Lee

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.