PMID: 3002430Oct 8, 1985

Biosynthesis of prolyl hydroxylase: evidence for two separate dolichol-media pathways of glycosylation

N L KedershaR A Berg


Prolyl hydroxylase is a glycoprotein containing two nonidentical subunits, alpha and beta. The alpha subunit of prolyl hydroxylase isolated from 13-day-old chick embryos contains a single high mannose oligosaccharide having seven mannosyl residues. Two forms of alpha subunit have been shown to exist in enzyme purified from tendon cells of 17-day-old chick embryos, one of which (alpha) appears to be identical in molecular weight and carbohydrate content with the single alpha of enzyme from 13-day-old chick embryos, as well as another form (alpha') that contains two oligosaccharides, each containing eight mannosyl units [see Kedersha, N. L., Tkacz, J. S., & Berg, R. A. (1985) Biochemistry (preceding paper in this issue)]. Biosynthetic labeling studies were performed with chick tendon cells using [2-3H]mannose, [6-3H]glucosamine, [14C(U)]mannose, and [14C(U)]glucose. Analysis of the labeled products using polyacrylamide gel electrophoresis in sodium dodecyl sulfate showed that only the oligosaccharides on alpha' incorporated measurable mannose or glucosamine isotopes; however, both alpha subunits incorporated 14C amino acid mix and [14C(U)]glucose [metabolically converted to [14C(U)]mannose] under similar conditions. Pulse-chase l...Continue Reading


Nov 22, 1976·Biochemical and Biophysical Research Communications·N A GuzmanD J Prockop
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·S Chen-KiangS Udenfriend
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Aug 1, 1990·Journal of Cellular Physiology·J A BassukR A Berg
Nov 19, 2003·European Journal of Biochemistry·Karl-Heinz HofbauerArmin Kurtz
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T HelaakoskiT Pihlajaniemi
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A BassukR A Berg
Jan 1, 1990·Annals of the New York Academy of Sciences·K I KivirikkoT Pihlajaniemi
May 1, 1988·Experimental Eye Research·J J Plantner, E L Kean

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