Biosynthesis of prostaglandin D2 by motoneurons and dorsal horn microneurons: a biochemical and high resolution immunocytochemical study in chick spinal cord

Neuroscience
M.-F. VesinB. Droz

Abstract

Prostaglandin D2 is one of the major prostanoids formed from [14C]arachidonic acid by the central nervous system. The aim of the present study is to specify the prostaglandin D2 biosynthetic capacity in the chick spinal cord and to identify the cell type involved in this synthesis. A highly specific and sensitive enzyme immunoassay allowed us to demonstrate that the amount of newly formed prostaglandin D2 increases proportionally with the concentration of free arachidonic acid of either exogenous or endogenous origin and reaches concentration values ranging from 10(-9) to 10(-6) M. The sites of prostaglandin D2 synthesis were localized in Vibratome sections of spinal cord after incubation with antibodies raised against glutathione-independent prostaglandin D synthase; controls were performed with anti-glutathione-dependent prostaglandin D synthase antibodies and non-immune rabbit or goat serum. After immunoprocessing, electron microscope examination revealed that the specific immunoreactivity was confined to small neurons of laminae II and III in the dorsal horn and to motoneurons in the ventral horn of the spinal cord. The immunodeposits were associated with rough endoplasmic reticulum profiles distributed throughout the dorsa...Continue Reading

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Citations

Aug 17, 1999·European Journal of Pharmacology·D H WrightA W Ford-Hutchinson
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