Biosynthesis of protein products by animal cells. Are growth and non-growth associated concepts valid or useful?

Cytotechnology
J P BarfordC Harbour

Abstract

The application of simple growth and non-growth associated concepts from microbial systems describing substrate uptake and production formation is considered unlikely to assist in the understanding of antibody formation and, hence, in maximising antibody yield. Such concepts have many significant limitations - notably, their strict application only to products of catabolic pathways and their inability to include metabolisms which either have multiple catabolic pathways (eg, fermentation and respiration in yeast and animal cells) or in which the major product of interest is predominantly anabolic in nature (eg. amino acid production in bacteria and antibody formation in animal cells). In addition, products which undergo an assembly and secretion process or a secretion process which allows intracellular pools of product to exist are also not well described by such simple relationships. In this work, inadequacies in the current approach to the study of the kinetics of growth of hybridoma cells and antibody production are described and the examples of growth ofSaccharomyces cerevisiae andCandida utilis, amino acid production by bacteria and antibody production by animal cells are used to illustrate these limitations. Having identif...Continue Reading

References

Jan 1, 1992·Cytotechnology·J P BarfordC Harbour
May 1, 1990·Bio/technology·C F Goochee, T Monica
Sep 1, 1991·Enzyme and Microbial Technology·K K Frame, W S Hu
Feb 5, 1989·Biotechnology and Bioengineering·N G RayP W Runstadler
Dec 20, 1989·Biotechnology and Bioengineering·E Suzuki, D F Ollis
Apr 15, 1990·Biotechnology and Bioengineering·J P Barford
Apr 15, 1990·Biotechnology and Bioengineering·J P Barford
Mar 5, 1992·Biotechnology and Bioengineering·R D Kiss, G Stephanopoulos
Aug 20, 1993·Biotechnology and Bioengineering·S K OhM G Yap
Feb 1, 1988·Cytotechnology·O W Merten

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Citations

Jun 19, 2008·Bioprocess and Biosystems Engineering·Penny DorkaJeno M Scharer
Jan 24, 2007·Bioprocess and Biosystems Engineering·Diliny M De AlwisMurray Moo-Young

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