Biosynthesis of Silver Nanoparticles Using Onion Endophytic Bacterium and Its Antifungal Activity against Rice Pathogen Magnaporthe oryzae

Journal of Fungi
Ezzeldin IbrahimBin Li

Abstract

Biosynthesis of silver nanoparticles (AgNPs) using endophytic bacteria is a safe alternative to the traditional chemical method. The purpose of this research is to biosynthesize AgNPs using endophytic bacterium Bacillus endophyticus strain H3 isolated from onion. The biosynthesized AgNPs with sizes from 4.17 to 26.9 nm were confirmed and characterized by various physicochemical techniques such as Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), UV-visible spectroscopy, transmission electron microscopy (TEM) and scanning electron microscopy (SEM) in addition to an energy dispersive spectrum (EDS) profile. The biosynthesized AgNPs at a concentration of 40 μg/mL had a strong antifungal activity against rice blast pathogen Magnaporthe oryzae with an inhibition rate of 88% in mycelial diameter. Moreover, the biosynthesized AgNPs significantly inhibited spore germination and appressorium formation of M. oryzae. Additionally, microscopic observation showed that mycelia morphology was swollen and abnormal when dealing with AgNPs. Overall, the current study revealed that AgNPs could protect rice plants against fungal infections.

References

Apr 6, 2002·Science·Donald Kennedy
Sep 18, 2002·Pest Management Science·Ulrich GisiAlan McCaffery
Aug 27, 2003·Medicinal Research Reviews·Li-June Ming
Oct 4, 2008·The Journal of Physical Chemistry. B·C CarlsonJ J Schlager
Feb 4, 2009·Trends in Biotechnology·Pari Skamnioti, Sarah J Gurr
Apr 5, 2012·Molecular Plant Pathology·Ralph DeanGary D Foster
Jun 21, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao GaoZhiqi Shi
Feb 20, 2019·International Journal of Molecular Sciences·Sang Hun Lee, Bong-Hyun Jun
May 28, 2020·Nanomaterials·Auriane Fifame Oussou-AzoMun'delanji Catherine M Vestergaard

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Citations

Aug 27, 2021·Journal of Fungi·Kamel A Abd-Elsalam

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Datasets Mentioned

BETA
MN611116

Methods Mentioned

BETA
PCR
electrophoresis
transmission
scanning
X-ray
Scanning Electron Microscopy
surface plasmon resonance

Software Mentioned

MEGA
SPSS
BLAST

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