Biosynthesis of soluble carotenoid holoproteins in Escherichia coli

Scientific Reports
Céline Bourcier de CarbonDiana Kirilovsky

Abstract

Carotenoids are widely distributed natural pigments that are excellent antioxidants acting in photoprotection. They are typically solubilized in membranes or attached to proteins. In cyanobacteria, the photoactive soluble Orange Carotenoid Protein (OCP) is involved in photoprotective mechanisms as a highly active singlet oxygen and excitation energy quencher. Here we describe a method for producing large amounts of holo-OCP in E.coli. The six different genes involved in the synthesis of holo-OCP were introduced into E. coli using three different plasmids. The choice of promoters and the order of gene induction were important: the induction of genes involved in carotenoid synthesis must precede the induction of the ocp gene in order to obtain holo-OCPs. Active holo-OCPs with primary structures derived from several cyanobacterial strains and containing different carotenoids were isolated. This approach for rapid heterologous synthesis of large quantities of carotenoproteins is a fundamental advance in the production of antioxidants of great interest to the pharmaceutical and cosmetic industries.

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Citations

May 11, 2016·Photosynthesis Research·E G MaksimovA B Rubin
Oct 25, 2016·Biochimica Et Biophysica Acta. Bioenergetics·Nikolai N SluchankoEugene G Maksimov
Apr 4, 2017·Database : the Journal of Biological Databases and Curation·Junko Yabuzaki
Sep 19, 2019·Nature Plants·Fernando MuzzopappaDiana Kirilovsky
Mar 11, 2018·Biochemistry. Biokhimii︠a︡·N N SluchankoE G Maksimov
Feb 19, 2017·Photosynthesis Research·Marcus MoldenhauerThomas Friedrich
Sep 19, 2020·Antioxidants·Eugene G MaksimovNikolai N Sluchanko
Jan 15, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Wenjing LouHaijun Liu
Apr 10, 2017·Current Opinion in Plant Biology·Han BaoCheryl A Kerfeld
Jun 23, 2019·Biochimica Et Biophysica Acta. Bioenergetics·Chakib DjediatCécile Bernard
Sep 20, 2015·Journal of the American Society for Mass Spectrometry·Hao ZhangMichael L Gross

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Datasets Mentioned

BETA
PCC6803

Methods Mentioned

BETA
protein folding
PCR

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