PMID: 6982958Dec 1, 1982Paper

Biosynthesis of somatostatin-like immunoreactivity by frog retinas in vitro

Journal of Neurochemistry
T Yamada, S Basinger

Abstract

Somatostatin-like immunoreactivity has been localized to a wide variety of central nervous system neurons, including the retina. We utilized the unique advantages the retina provides for in vitro studies of nerves to examine the biosynthesis of somatostatin. Extracts of frog retinas pulse-labeled with [35S]cysteine for various time periods revealed uptake of radioactivity into material adsorbable by anti-somatostatin antibody linked to affinity beads. This uptake increased in a curvilinear fashion for 4 h and was inhibited by cycloheximide (0.2 mM) or by boiling the retinas prior to labeling. Pulse-chase experiments revealed that affinity-adsorbable radioactivity from retinal extracts decreased with time of incubation in chase medium; 89% of this decrease could be accounted for by increased in the affinity-adsorbable radioactivity of the chase medium. Chromatography of the retinal extracts on Sephadex G50 (superfine) revealed four elution peaks, whereas only one peak, co-eluted with somatostatin-14, could be identified in the medium. Chromatographic elution patterns of affinity-adsorbable radioactivity from extracts of pulse-labeled retinas incubated in chase medium for various times showed a gradual shift of radioactivity from...Continue Reading

References

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Citations

Mar 5, 1984·Life Sciences·A E PekaryJ M Hershman
Oct 1, 1992·Molecular and Cellular Endocrinology·B ColásE Arilla
Feb 1, 1988·The Journal of Clinical Investigation·T ChibaT Yamada
Sep 1, 1983·Journal of Neurochemistry·D W MarshakT Yamada
Jul 13, 2000·Microscopy Research and Technique·J JohnsonN C Brecha
May 1, 1984·The Journal of Comparative Neurology·D W MarshakW K Stell

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