PMID: 3763437Jul 1, 1986Paper

Biosynthetic fate of the amino-terminal fragment of pro-opiomelanocortin within the intermediate lobe of the mouse pituitary

Peptides
H P Bennett

Abstract

All the biosynthetic derivatives of pro-opiomelanocortin (POMC) were purified from an extract of 300 mouse neurointermediate pituitaries. Inspection of the amino acid composition of these peptides indicated that cleavage at all available dibasic processing sites within POMC was essentially complete except for -Arg49-Lys50- within the 1 to 74 amino-terminal sequence. Only about 50% of the 1 to 74 fragment was processed to the 1 to 49 sequence and Lys1 gamma 3MSH (i.e., the 50 to 74 sequence). The existence of these derivatives of the 1 to 74 fragment was confirmed by pulse-labelling explant cultures of mouse neurointermediate pituitaries with tritiated amino acids. Pulse/chase biosynthetic experiments indicated that the cleavage of the 1 to 74 sequence takes place 3 to 6 hours post-translation. This time course of biosynthesis suggests that the cleavage of the 1 to 74 sequence is a secretory granule event. Time course studies revealed that the minimum time required for newly synthesized derivatives of POMC to emerge from the intermediate lobe tissue was approximately 3 hours.

References

Jan 28, 1981·Molecular and Cellular Biochemistry·M Chrétien, N G Seidah
Jul 2, 1982·Science·H HoshinaI Boime
Jul 1, 1983·The Journal of Endocrinology·B G JenksA P van Overbeeke
May 15, 1981·Biochemical and Biophysical Research Communications·C A BrowneS Solomon

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Citations

Mar 7, 1995·Regulatory Peptides·P RobinsonH P Bennett
Nov 2, 2015·Domestic Animal Endocrinology·Z-Q WangY-X Tao
Jul 8, 2008·Journal of Neuroendocrinology·A B Bicknell
Nov 1, 1990·Trends in Endocrinology and Metabolism : TEM·R E Mains, B A Eipper
Feb 1, 1990·Bioscience Reports·N J Darby, D G Smyth
Oct 28, 2008·Molecular and Cellular Endocrinology·David J Pepper, Andrew B Bicknell
Apr 1, 2015·PeerJ·Fereshteh T YazdiDavid Meyre
Feb 7, 2006·Cell Metabolism·Heike BiebermannHeiko Krude
Feb 18, 2016·Journal of Molecular Endocrinology·Niamh X CawleyY Peng Loh
Aug 30, 2018·Physiological Reviews·Erika HarnoAnne White
Jul 19, 2019·Frontiers in Medicine·Anna Elizabeth MoscowitzMehdi Mirsaeidi
Jun 26, 2020·Biochimica Et Biophysica Acta. General Subjects·Avik ShomeKathleen G Mountjoy
May 3, 2005·General and Comparative Endocrinology·Robert M Dores, Stephanie Lecaude

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