Biosynthetic pathway leading to gentamicin C2b.

Antimicrobial Agents and Chemotherapy
B K LeeJ A Waitz

Abstract

Incubation of Micromonospora purpurea SC 1210 (NRRL 5467) with L-[methyl-14C]methionine yielded [methyl-14C]gentamicin A and methyl-14C-labeled antibiotic JI-20A in a molar radioactivity ratio of 9:2. We did not isolate methyl-14C-labeled gentamicin X2, which was expected as an intermediate based on the biosynthetic pathways proposed by others. Addition of methyl-14C-labeled antibiotic JI-20A to M. purpurea SC 1124 (NRRL 8102) yielded [methyl-14C]-gentamicin C1a and [methyl-14C]gentamicin C2b in variable molar radioactivity ratios. These data do not support the biosynthetic pathway leading to gentamicin C2b proposed by Testa and Tilley.

References

Nov 1, 1977·The Journal of Antibiotics·T DeguchiM Tanaka
Sep 1, 1977·Antimicrobial Agents and Chemotherapy·B K LeeM J Weinstein
Feb 1, 1976·The Journal of Antibiotics·R T Testa, B C Tilley
Dec 1, 1973·The Journal of Antibiotics·B K LeeC Schaffner

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