PMID: 799246Dec 22, 1976Paper

Biosynthetic regulation of individual proteins in relA+ and relA strains of Escherichia coli during amino acid starvation

Molecular & General Genetics : MGG
S ReehJ D Friesen

Abstract

An isogenic pair of Escherichia coli mutants (relA+ tufB valSts and relA1 tufB valSts) has been cultured at several temperatures to establish various degrees of limitation for valyl-tRNA synthetase. The biosynthetic rate of 16 identifiable proteins, most of which are components of the transcription and translation apparatus, was measured by pulse-labelling with [35S]-methionine, followed by protein separation using two-dimensional gel electrophoresis (O'Farrell, 1975). No single pattern of response to amino acid starvation of biosynthetic rate was observed. EF-Ts, L12 and S6 were found to be under strong stringent and relaxed regulation; EF-G, EF-Tu-A and S1 are under strong stringent, but weak relaxed regulation; EF-Tu-B, alpha, VRS, IRS and ARS are under week stringent and weak relaxed regulation; beta is under weak stringent regulation and does not respond at all to relaxed conditions; the biosynthetic rate of a protein called stringent starvation protein is strongly stimulated, relative to other proteins, in the starved stringent strain.

References

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Citations

May 23, 1979·Molecular & General Genetics : MGG·N P FiilJ D Friesen
Jan 1, 1980·Molecular & General Genetics : MGG·J BaluchJ Resnick
Feb 1, 1980·Molecular & General Genetics : MGG·J Parker, J D Friesen
Jun 7, 1979·Molecular & General Genetics : MGG·S Reeh, S Pedersen
Jan 1, 1984·Molecular & General Genetics : MGG·S PedersenA Ishihama
Feb 26, 1979·Molecular & General Genetics : MGG·M ShibuyaY Kaziro
Dec 1, 1986·Molecular & General Genetics : MGG·A K Ulrich, J Parker
Feb 7, 1978·Molecular & General Genetics : MGG·P G Lemaux, D L Miller
Jan 1, 1980·Molecular & General Genetics : MGG·J C PatteF Borne
Jan 1, 1985·Molecular & General Genetics : MGG·R FukudaH Matsubara
Dec 3, 1981·Nature·L HudsonA Landy
Nov 1, 1991·The Plant Journal : for Cell and Molecular Biology·Y TakahashiT Nagata
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Yamamoto, M Nomura
Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·L E PostP P Dennis
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S PeacockH Weissbach
Jun 11, 1985·Nucleic Acids Research·D BedwellL Lindahl
Feb 11, 1987·Nucleic Acids Research·H Serizawa, R Fukuda
Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Jun 27, 2013·Critical Reviews in Microbiology·Bhaskar Chandra Mohan RamisettyRamachandran Sarojini Santhosh
Jan 1, 1980·Biochimie·A J Cozzone

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