Biosynthetically lipid-modified human scFv fragments from phage display libraries as targeting molecules for immunoliposomes

FEBS Letters
J de KruifT Logtenberg

Abstract

A human anti-CD22 single chain (sc) Fv antibody fragment from a synthetic phage antibody display library was biosynthetically lipid-tagged by using Escherichia coli lipoprotein sequences. The purified anti-CD22 scFv lipoprotein was incorporated into liposomes by detergent dilution. Anti-CD22 immunoliposomes were shown to bind specifically in a dose- and time-dependent manner to CD22+ cell lines and CD22+ B-lymphocytes present in freshly isolated samples of blood mononuclear cells. The immunoliposomes were demonstrated to accumulate in intracellular compartments. Biosynthetically lipid-tagged human scFv antibody fragments isolated from phage display libraries may facilitate the construction of immunoliposomes with improved properties.

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Citations

Sep 1, 2000·Advanced Drug Delivery Reviews·F NilssonD Neri
Jun 6, 2000·Advanced Drug Delivery Reviews·E MastrobattistaG Storm
Sep 1, 1999·European Journal of Clinical Investigation·H J BloemendalE E Voest
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Jan 31, 2018·Nature Nanotechnology·Ranit KedmiDan Peer
Oct 31, 2018·Nature Communications·Nuphar VeigaDan Peer
Jul 5, 2001·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·G Bendas

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