Abstract
Acinetobacter baumannii has been well recognized as a problematic human pathogen and several reports has shown the incidence of multidrug and pandrug-resistant A. baumannii strains in infirmary infections. A. baumannii grows only on an air-liquid interface and does not form a contiguous biofilm. Extracellular matrices (ECM) and slanted glass coverslips are (SGC) used as biofilm substrates and biofilms have been investigated by SEM, confocal and crystal violet staining. ECM has shown enhanced biofilm formation under dynamic conditions rather than static conditions. SGC biofilm yield assay has shown higher levels of continuous layers and packed thicker biofilm formation with glass coverslip inserts, up to 1.7 to 3 times higher biofilm formation, than when compared with no glass coverslip inserts. A media immersed ECM study revealed that biofilm grown on extracellular matrixes formed thread-like pili structures, and that these structures had contact with the ECM and also showed excellent cell-to-cell interaction. In summary, A. baumannii showed higher biofilm formation capacities with ECM, while the prominent results were directly related with the biofilm formation capacity of A. baumannii. For the initial step of biofilm formatio...Continue Reading
References
Jan 1, 1983·Annual Review of Microbiology·W Harder, L Dijkhuizen
Aug 1, 1996·Thorax·P M GamboaV Múgica
Jun 1, 1997·Journal of Clinical Microbiology·C WendtH Rüden
Mar 25, 1998·Journal of Medical Microbiology·S P HawserL J Douglas
Jul 3, 1998·Journal of Clinical Microbiology·A JawadP M Hawkey
Jan 16, 1999·Advances in Microbial Physiology·M P Spector
Mar 30, 1999·Research in Microbiology·M VaneechoutteL Dijkshoorn
May 21, 1999·Science·J W CostertonE P Greenberg
Jun 5, 1999·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J BerlauT Pitt
Nov 1, 2000·Epidemiology and Infection·H M AuckenT L Pitt
Jun 1, 2002·Applied and Environmental Microbiology·D DjordjevicL A McLandsborough
Nov 6, 2003·Journal of Electron Microscopy·Juliana C AraujoRosana F Vazoller
Dec 10, 2003·Microbiology·Andrew P TomarasLuis A Actis
Jul 2, 2005·Current Microbiology·Swarajit K Biswas, W LaJean Chaffin
Dec 6, 2005·Research in Microbiology·Je Chul LeeLenie Dijkshoorn
Feb 13, 2007·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Gislaine Franco de Moura CostaLourdes Botelho Garcia
May 22, 2007·Microbial Pathogenesis·Roberta Rubesa MihaljevicMaja Abram
Nov 17, 2007·Nature Reviews. Microbiology·Lenie DijkshoornHarald Seifert
Nov 1, 2008·Indian Journal of Medical Microbiology·R Srinivasa RaoK Prashanth
Jul 11, 2009·Journal of Medical Microbiology·Anke KohlenbergHarald Seifert
Jan 1, 2014·Antimicrobial Agents and Chemotherapy·Jeng Yeong ChowWen Shan Yew
Mar 25, 2014·Current Opinion in Microbiology·Cristina SolanoIñigo Lasa
Apr 19, 2015·BMC Microbiology·Li-mei LuoYi Xie
Feb 24, 2016·Molecular Psychiatry·L SchmaalD P Hibar