BioTimer Assay, a new method for counting Staphylococcus spp. in biofilm without sample manipulation applied to evaluate antibiotic susceptibility of biofilm

Journal of Microbiological Methods
Fabrizio PantanellaFrancesca Berlutti

Abstract

The medical device-related infections are frequently a consequence of Staphylococcus biofilm, a lifestyle enhancing bacterial resistance to antibiotics. Antibiotic susceptibility tests are usually performed on planktonic forms of clinical isolates. Some methods have been developed to perform antibiotic susceptibility tests on biofilm. However, none of them counts bacterial inoculum. As antibiotic susceptibility is related to bacterial inoculum, the test results could be mistaken. Here, a new method, BioTimer Assay (BTA), able to count bacteria in biofilm without any manipulation of samples, is presented. Moreover, the BTA method is applied to analyze antibiotic susceptibility of six Staphylococcus strains in biofilm and to determine the number of viable bacteria in the presence of sub-inhibitory doses of four different antibiotics. To validate BTA, the new method was compared to reference methods both for counting and antibiotic susceptibility tests. A high agreement between BTA and reference methods is found on planktonic forms. Therefore, BTA was employed to count bacteria in biofilm and to analyze biofilm antibiotic susceptibility. Results confirm the high resistance to antibiotics of Staphylococcus biofilm. Moreover, BTA co...Continue Reading

References

Oct 1, 1987·Antimicrobial Agents and Chemotherapy·B L ProsserR Cleeland
Mar 21, 2001·Antimicrobial Agents and Chemotherapy·K Lewis
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Jun 6, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J Carratalà
Oct 1, 2003·Journal of Biomedical Materials Research. Part a·Francesca BerluttiPiera Valenti
Mar 16, 2005·Pharmacotherapy·Megan B Bestul, Heather L Vandenbussche
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Robin K PettitMarilyn L Horton
Feb 9, 2006·The Journal of Antimicrobial Chemotherapy·Jonathan A T SandoeMark H Wilcox
Sep 26, 2006·Applied and Environmental Microbiology·Liron SilbertRaz Jelinek
Oct 25, 2006·Antimicrobial Agents and Chemotherapy·Maria EgervärnSven Lindgren
Jan 27, 2007·The Journal of Antimicrobial Chemotherapy·Matthew E FalagasIoannis Chatzinikolaou
Dec 25, 2007·Journal of Microbiological Methods·Elke PeetersTom Coenye

❮ Previous
Next ❯

Citations

Dec 21, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Fatma Kaynak OnurdağUfuk Abbasoğlu
Nov 4, 2010·The Journal of Microbiology·Shukho KimJungmin Kim
Sep 21, 2015·Biotechnology Letters·Shilpi Srivastava, Atul Bhargava
Aug 6, 2011·The Journal of Surgical Research·Donavon J HessCarol L Wells
Jul 13, 2013·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Yanling CaiKen Welch
Mar 5, 2014·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Umberto RomeoFrancesca Berlutti
Oct 19, 2011·Interdisciplinary Perspectives on Infectious Diseases·Fabrizio PantanellaPiera Valenti
Oct 15, 2010·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·U LorenzR Kellersmann
Jan 1, 2012·Journal of Functional Biomaterials·Ken WelchMaria Strømme
Mar 15, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Samuel C Uzoechi, Nehal I Abu-Lail
Jan 20, 2011·International Journal of Immunopathology and Pharmacology·F BerluttiA Polimeni
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Jan 1, 2014·3 Biotech·Yanling CaiKen Welch
Oct 4, 2017·NPJ Biofilms and Microbiomes·Mathias MüskenSusanne Häussler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.