Biotin staining in the giant fiber systems of the lobster

The Journal of Comparative Neurology
P M Ma

Abstract

The avidin-biotin-complex method is a popular immunocytochemical technique. This method labels consistently a group of neurons in the lobster ventral nerve cord in the absence of primary antibodies. The specific staining is due to a relatively high level of endogenous biotin (or biocytin) in these neurons. These biotin-positive neurons are located in the supraesophageal, thoracic, and abdominal ganglia. Intraaxonal injection of Lucifer yellow followed by Texas red-conjugated streptavidin staining reveals that the neurons are members of the medial giant (MG) and lateral giant (LG) systems, which are important in mediating rapid tail flipping during escape maneuvers. In neuronal somata, staining is restricted to the cytoplasm. Within MG axons, staining appears as punctate, subaxolemmal structures. Preincubating nerve cords in biocytin or direct intraaxonal injection of biocytin enhances staining of these punctate organelles. In LG axons, staining is localized to fragments of braided filamentous structures that also appear to be associated with the axolemma. Preincubation of ventral nerve cords in various concentrations of biocytin results in the appearance of additional groups of stained neurons, suggesting that there are subsets...Continue Reading

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Citations

May 6, 1996·The Journal of Comparative Neurology·S EisnerF Scalia
Nov 15, 1996·Clinica Chimica Acta; International Journal of Clinical Chemistry·J Hymes, B Wolf
Apr 7, 2004·The Journal of Comparative Neurology·Bruce E McKayRay W Turner
Oct 27, 2015·Frontiers in Zoology·Konrad StolzJohannes Strauß

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