Biotin transport and accumulation by cells of Lactobacillus plantarum. I. General properties of the system.

Journal of Bacteriology
J R Waller, H C LICHSTEIN

Abstract

Waller, James R. (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Biotin transport and accumulation by cells of Lactobacillus plantarum. I. General properties of the system. J. Bacteriol. 90:843-852. 1965.-Resting cells of Lactobacillus plantarum were saturated with bound biotin by incubation in phosphate buffer with biotin and glucose for 2 hr. This bound biotin was stable to wide changes in temperature, pH, and reaction time. Free biotin could be eluted from the cells by suspending them in cold water or saline. Immersing the cells in boiling water for 30 sec released all free biotin. Recoveries of added biotin exceeded 92%. Free biotin uptake by bound biotin-saturated cells occurred by two mechanisms. One process was independent from temperature (Q(10), 1.25), pH, cellular metabolism, and inhibition by iodoacetate. The other mechanism was dependent upon temperature (Q(10), 2.58; optimum, 37 C), pH (optimum, 7.5), and active cellular metabolism, and was inhibited by iodoacetate. Activation energies of 3,700 and 13,800 cal per mole, respectively, were observed for glucose-independent and -dependent free biotin uptake. Both processes exhibited approximately the same degree of inhibition by homobiotin. Highe...Continue Reading

References

Sep 1, 1957·Bacteriological Reviews·G N COHEN, J MONOD
Feb 1, 1959·The Biochemical Journal·M BURGERA KLEINZELLER
Jan 1, 1960·Advances in Protein Chemistry·H N CHRISTENSEN
Jan 1, 1961·Journal of Bacteriology·H C LICHSTEIN, J R WALLER
Nov 1, 1960·Journal of Bacteriology·J T WACHSMAN, R STORCK
May 1, 1962·Journal of Bacteriology·R J HARVEY, E B COLLINS
Sep 1, 1962·Bacteriological Reviews·R J BRITTEN, F T McCLURE
Dec 1, 1962·Journal of Bacteriology·V P CIRILLO
May 6, 1960·Biochimica Et Biophysica Acta·A KEPES
Aug 15, 1959·Nature·K OVERGAARD-HANSEN, U V LASSEN
Jan 1, 1962·Proceedings of the Society for Experimental Biology and Medicine·J A JACQUEZ
Dec 19, 1941·Science·R C ThompsonR J Williams

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Citations

Jan 1, 1980·Journal of Supramolecular Structure·G Cecchini, E B Kearney
Jan 1, 1978·Journal of Bacteriology·J F Cicmanec, H C Lichstein
Sep 1, 1972·Biochimica Et Biophysica Acta·J M Becker, H C Lichstein
May 21, 1982·Biochimica Et Biophysica Acta·A PiffeteauM Gaudry
Aug 5, 1975·Biochimica Et Biophysica Acta·T ViswanathaM Wilchek
Nov 1, 1969·Journal of Bacteriology·T O Rogers, H C Lichstein
Oct 1, 1974·Journal of Bacteriology·N Hasan, I F Durr
Nov 1, 1974·Journal of Bacteriology·O Prakash, M A Eisenberg
Jul 1, 1971·Applied Microbiology·G M Fillipi, J W Vennes
Dec 1, 1990·Biochemical Medicine and Metabolic Biology·D Weiner, B Wolf
Jul 1, 1969·Archives of Biochemistry and Biophysics·J Birnbaum
Jun 24, 1980·Biochemistry·A PiffeteauA Marquet
Oct 29, 1982·Biochemical and Biophysical Research Communications·N D Cohen, M Thomas

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