Biotin transport and accumulation by cells of Lactobacillus plantarum. II. Kinetics of the system.

Journal of Bacteriology
J R Waller, H C LICHSTEIN

Abstract

Waller, James R. (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Biotin transport and accumulation by cells of Lactobacillus plantarum. II. Kinetics of the system. J. Bacteriol. 90:853-856. 1965.-Bound biotin-saturated cells of Lactobacillus plantarum accumulated free biotin by a time-dependent process exhibiting substrate saturation phenomena in the presence and absence of glucose. Apparent K(m) and V(max) values determined in the presence and absence of glucose, respectively, from Lineweaver-Burk plots were found to be 31.5 and 7.72 mmum (K(m)) and 9.72 and 3.26 mumumoles per mg per min (V(max)). Free biotin transport per se appeared to be an energy-independent, mediated process, whereas the accumulation of large intracellular vitamin concentrations was energy-dependent. Internal free biotin was quantitatively converted to bound biotin. The rate and extent of bound biotin formation was slower than free biotin uptake, and dependent upon intracellular free biotin levels up to a saturating concentration.

References

Jun 28, 1961·Biochemical and Biophysical Research Communications·D P KOSOW, M D LANE
Jan 1, 1961·Journal of Bacteriology·H C LICHSTEIN, J R WALLER
Sep 1, 1962·Journal of Bacteriology·V P CIRILLO
May 6, 1960·Biochimica Et Biophysica Acta·A KEPES

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Citations

Feb 9, 2020·Biotechnology and Bioengineering·Julius Laurens RomboutsMark Cornelis Maria van Loosdrecht
May 21, 1982·Biochimica Et Biophysica Acta·A PiffeteauM Gaudry
Aug 5, 1975·Biochimica Et Biophysica Acta·T ViswanathaM Wilchek
Nov 1, 1969·Journal of Bacteriology·T O Rogers, H C Lichstein
Nov 1, 1974·Journal of Bacteriology·O Prakash, M A Eisenberg
Dec 1, 1990·Biochemical Medicine and Metabolic Biology·D Weiner, B Wolf
Oct 29, 1982·Biochemical and Biophysical Research Communications·N D Cohen, M Thomas

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