Biotransformation of 2,4,6-trinitrotoluene (TNT) by enchytraeids (Enchytraeus albidus) in vivo and in vitro

Environmental Pollution
Sabine G DodardGeoffrey I Sunahara

Abstract

2,4,6-Trinitrotoluene (TNT) is toxic to soil invertebrates, but little is known about its toxicokinetic behavior in soil. Tissue residue analysis was used to evaluate whether the presence of TNT and its reduced metabolites in soil invertebrates was due to uptake of these compounds from the soil into the organism, or due to microbial transformation of TNT associated with the organism followed by uptake. Adult white potworms (Enchytraeus albidus) were exposed to non-lethal concentrations of TNT in amended artificial soil for 21 d, or to TNT in solution for 20 h. Soil exposure studies confirmed earlier reports that TNT was transformed in enchytraeids in vivo to 2- and 4-aminodinitrotoluenes. However, enchytraeid exposure to TNT in solution led to the additional presence of 2,4-diaminonitrotoluene as well as 2- and 4- hydroxyamino-dinitrotoluenes and azoxy-compounds, suggesting that TNT can be metabolized in vivo in the absence of soil. Incubation of unexposed enchytraeid homogenates with TNT led to a protein-dependent appearance of these metabolites in vitro after > or =16 h incubation. Cellular fractionation studies indicated that most of this activity resided in the 8000 x g pellet, and was completely inhibited by broad-spectrum...Continue Reading

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Citations

Aug 2, 2005·Archives of Environmental Contamination and Toxicology·G R Lotufo, M J Lydy
Sep 12, 2013·Journal of Hazardous Materials·Sabine G DodardGeoffrey I Sunahara
Dec 6, 2005·Chemosphere·Alethea T BowenThomas W La Point
Sep 15, 2005·Health Economics·George FranceAndrea Donatini
Mar 12, 2010·Ecotoxicology and Environmental Safety·Guilherme R LotufoAlfreda B Gibson

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