Biotransformation of 6,6-Dimethylfulvene by Pseudomonas putida RE213.

Applied and Environmental Microbiology
R W Eaton, S A Selifonov

Abstract

The biotransformation of 6,6-dimethylfulvene [5-(1-methylethylidene)-1,3-cyclopentadiene], a nonaromatic C(inf5) carbocyclic analog of isopropylbenzene, was examined by using Pseudomonas putida RE213, a Tn5-generated dihydrodiol-accumulating mutant of the isopropylbenzene-degrading strain P. putida RE204. 6,6-Dimethylfulvene was converted to a single chiral product identified as (+)-(1R,2S)-cis-1,2-dihydroxy-5-(1-methylethylidene)-3-cyclopentene. This isopropylbenzene 2,3-dioxygenase-catalyzed transformation demonstrates the potential of bacterial arene dioxygenases for the direct conversion of cyclopentadienylidene compounds to homochiral C(inf5) carbocyclic cis-diols for use in enantiocontrolled organic syntheses.

References

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Jul 1, 1988·Applied and Environmental Microbiology·L P Wackett, D T Gibson
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Nov 1, 1993·Applied and Environmental Microbiology·C H Nakatsu, R C Wyndham

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Citations

Mar 29, 2001·Chirality·J Baumgartner, H Weber

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