Biotransformation of Panax notoginseng saponins into ginsenoside compound K production by Paecilomyces bainier sp. 229

Journal of Applied Microbiology
W ZhouP Zhou

Abstract

Development and optimization of an efficient and inexpensive biotransformation process for ginsenoside compound K production by Paecilomyces bainier sp. 229. We have determined the optimum culture conditions required for the efficient production of ginsenoside compound K by P. bainier sp. 229 via biotransformation of ginseng saponin substrate. The optimal medium constituents were determined to be: 30 g sucrose, 30 g soybean steep powder, 1 g wheat bran powder, 1 g (NH(4))(2)SO(4), 2 g MgSO(4) x 7H(2)O and 1 g CaCl(2) in 1 l of distilled water. An inoculum size of 5-7.5% with an optimal pH range of 4.5-5.5 was essential for high yield. The Mol conversion quotient of ginseng saponins increased from 21.2% to 72.7% by optimization of the cultural conditions. Scale-up in a 10 l fermentor, under conditions of controlled pH and continuous air supply in the optimal medium, resulted in an 82.6% yield of ginsenoside compound K. This is the first report on the optimization of culture conditions for the production of ginsenoside compound K by fungal biotransformation. The degree of conversion is significantly higher than previous reports. Our method describes an inexpensive, rapid and efficient biotransformation system for the production o...Continue Reading

References

Oct 1, 1996·Planta medica·H HasegawaM Uchiyama
Jun 10, 1998·Biochemical and Biophysical Research Communications·C WakabayashiI Saiki
Jun 26, 2002·Biological & Pharmaceutical Bulletin·Eun-Ah BaeDong-Hyun Kim
Jul 18, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Mona Abdel TawabManfred Schubert-Zsilavecz
Sep 16, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Hae-Ung LeeDong-Hyun Kim
Nov 23, 2005·Biopharmaceutics & Drug Disposition·In Bok PaekHye Suk Lee
Mar 17, 2006·Acta Pharmacologica Sinica·Min Han, Xiao-Ling Fang

❮ Previous
Next ❯

Citations

Apr 9, 2010·Applied Microbiology and Biotechnology·Chang-Su ParkDeok-Kun Oh
Nov 6, 2010·Applied Microbiology and Biotechnology·Mi-Hyun YooDeok-Kun Oh
Feb 10, 2009·Bioscience, Biotechnology, and Biochemistry·Kyeong-Hwan NohDeok-Kun Oh
Jan 25, 2011·Critical Reviews in Microbiology·V H TournasE J Katsoudas
Sep 19, 2015·Critical Reviews in Biotechnology·Kyung-Chul Shin, Deok-Kun Oh
Apr 24, 2012·Phytochemistry·Dimitre A Ivanov, Mark A Bernards
Dec 3, 2014·Fitoterapia·Xi-Ding YangGuo-Ping Yang
Oct 30, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei-Nan WangZhi-Dong Qiu
Nov 2, 2016·Food & Function·Jisun Oh, Jong-Sang Kim
Mar 27, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wen-Fang LiKe-Ke Li
Feb 15, 2020·International Journal of Molecular Sciences·Kyung-Chul ShinYeong-Su Kim
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yun-Yan KuangWei Zhou
Feb 14, 2019·Journal of Agricultural and Food Chemistry·Dashuai LiWenyu Lu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.