Biotransformation of steroids by entomopathogenic strains of Isaria farinosa

Microbial Cell Factories
Ewa KozłowskaTomasz Janeczko

Abstract

Steroid compounds are very interesting substrates for biotransformation due to their high biological activity and a high number of inactivated carbons which make chemical modification difficult. Microbial transformation can involve reactions which are complicated and uneconomical in chemical synthesis, and searching for a new effective biocatalyst is necessary. The best known entomopathogenic species used in steroid modification is Beauveria bassiana. In this study we tested the ability of Isaria farinosa, another entomopathogenic species, to transform several steroids. Twelve strains of the entomopathogenic filamentous fungus Isaria farinosa, collected in abandoned mines located in the area of the Lower Silesian Voivodeship, Poland, from insects' bodies covered by fungus, were used as a biocatalyst. All the tested strains effectively transformed dehydroepiandrosterone (DHEA). We observed 7α- and 7β-hydroxy derivatives as well as changes in the percentage composition of the emerging products. Due to the similar metabolism of DHEA in all tested strains, one of them was selected for further investigation. In the culture of the selected strain, Isaria farinosa KCh KW1.1, transformations of androstenediol, androstenedione, adrenost...Continue Reading

References

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Citations

Sep 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Monika DymarskaEdyta Kostrzewa-Susłow
Jun 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Monika DymarskaEdyta Kostrzewa-Susłow
Feb 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qunfang WengQiongbo Hu
Jul 26, 2019·Scientific Reports·Ewa KozłowskaTomasz Janeczko
Sep 9, 2018·Scientific Reports·Ewa KozłowskaTomasz Janeczko
Jul 22, 2019·Applied and Environmental Microbiology·Wei LuDunming Zhu
Jul 23, 2020·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Qianqian LiShaofeng Rong
Sep 1, 2021·Applied Microbiology and Biotechnology·Sushma MishraNamrata Singh

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Datasets Mentioned

BETA
AB080087.1

Methods Mentioned

BETA
PCR
NMR

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