Biotransformation of sucrose into hexyl-α-D-glucopyranoside and -polyglucosides by whole cells of Microbacterium paraoxydans

Biotechnology Letters
Shivkesh DahiyaSaroj Mishra

Abstract

To determine the transglycosylation activity of cell-bound enzymes from Microbacterium paraoxydans to catalyze the synthesis of hexyl-α-D-glucoside (HG) and -polyglucosides using sucrose as a glycosyl donor. Maximum HG yield (14.8 %) was achieved at 0.96 water activity in 12 h with sucrose at 0.5 M with lyophilized cells (equivalent to 8 IU α-glucosidase activity). The synthesized alkyl-glucosides and-polyglucosides were characterized by ESI-MS. Structural elucidation of the main product (purified by solid phase chromatography) was done by HSQC (2D NMR) which was confirmed as 1-hexyl-α-D-glucopyranoside. The synthesis was scaled up in a fed-batch reactor, with continuous feeding of whole cells every 6 h and a total yield of ~44 % was obtained for hexyl-glucoside and -polyglucosides under the optimized conditions. Synthesis of HG, hexyl di- and tri-glucosides has been achieved using a novel method.

References

May 28, 2004·Journal of Biotechnology·Sapna Das-BradooRajni Hatti-Kaul
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Kuniki KinoKohtaro Kirimura
May 22, 2009·Biotechnology Letters·Young-Min KimDoman Kim
Jul 18, 2009·Biotechnology and Bioengineering·David SvenssonPatrick Adlercreutz
Apr 13, 2013·Applied Microbiology and Biotechnology·Swati OjhaSubhash Chand

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Citations

Feb 14, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mpho S MafaBrett I Pletschke

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