Biotransformations catalyzed by cloned p-cymene monooxygenase from Pseudomonas putida F1

Applied Microbiology and Biotechnology
T NishioP C Lau

Abstract

p-Cymene monooxygenase (CMO) from Pseudomonas putida F1 consists of a hydroxylase (CymA1) and a reductase component (CymA2) which initiate pcymene (p-isopropyltoluene) catabolism by oxidation of the methyl group to p-isopropylbenzyl alcohol (p-cumic alcohol). To study the possible diverse range of substrates catalyzed by CMO, the cymA1A2 genes were cloned in an Escherichia coli pT7-5 expression system and the cells were used in transformation experiments. The tested substrates include different substituents on the aromatic ring at the 2 (ortho), 3 (meta) or 4 (para) position relative to the methyl moiety. As a result, a distinct preference was observed for substrates containing at least an alkyl or heteroatom substituent at the para-position of toluene. The conversion rate of 4-chlorotoluene or 4-methylthiotoluene to the corresponding benzyl alcohol was found to be as good as the canonical substrate, p-cymene. But 3-chlorotoluene, 4-fluorotoluene and 4-nitrotoluene were relatively poor substrates. CMO is also capable of producing styrene oxide from styrene. However, the oxidation of 4-chlorostyrene to 4-chlorostyrene oxide was by far the fastest among the substrates used in this study. The various biotransformation products wer...Continue Reading

Citations

Jul 11, 2006·Applied Microbiology and Biotechnology·Aisling MooneyKevin E O'Connor
Aug 27, 2011·Applied Microbiology and Biotechnology·Daniel Dobslaw, Karl-Heinrich Engesser
Jun 1, 2002·Current Opinion in Chemical Biology·Zhi LiBernard Witholt
Dec 25, 2013·Environmental Science & Technology·Yongming ZhangBruce E Rittmann
Mar 19, 2014·Bioresource Technology·Niti B JadejaAtya Kapley

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