PMID: 9192622Jun 24, 1997Paper

Bipartite substrate discrimination by human nucleotide excision repair

Proceedings of the National Academy of Sciences of the United States of America
M T HessHanspeter Naegeli

Abstract

Mammalian nucleotide excision repair (NER) eliminates carcinogen-DNA adducts by double endonucleolytic cleavage and subsequent release of 24-32 nucleotide-long single-stranded fragments. Here we manipulated the deoxyribose-phosphate backbone of DNA to analyze the mechanism by which damaged strands are discriminated as substrates for dual incision. We found that human NER is completely inactive on DNA duplexes containing single C4'-modified backbone residues. However, the same C4' backbone variants, which by themselves do not perturb complementary hydrogen bonds, induced strong NER reactions when incorporated into short segments of mispaired bases. No oligonucleotide excision was detected when DNA contained abnormal base pairs without concomitant changes in deoxyribose-phosphate composition. Thus, neither C4' backbone lesions nor improper base pairing stimulated human NER, but the combination of these two substrate alterations constituted an extremely potent signal for double DNA incision. In summary, we used C4'-modified backbone residues as molecular tools to dissect DNA damage recognition by human NER into separate components and identified a bipartite discrimination mechanism that requires changes in DNA chemistry with concu...Continue Reading

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J C HuangA Sancar
Aug 1, 1992·Molecular Microbiology·J J Lin, A Sancar
Jan 1, 1991·Annual Review of Genetics·P Modrich
Jan 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M S AkhlaqC von Sonntag
Jul 7, 1995·The Journal of Biological Chemistry·A Sancar
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J H Hoeijmakers
Feb 10, 1995·The Journal of Biological Chemistry·D MuA Sancar
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·J C HuangA Sancar
Jan 1, 1994·Environmental and Molecular Mutagenesis·P C Hanawalt
Apr 5, 1996·The Journal of Biological Chemistry·D MuA Sancar
Jan 1, 1996·Annual Review of Biochemistry·A Sancar
Feb 25, 1997·Biochemistry·M T HessH Naegeli

❮ Previous
Next ❯

Citations

Jun 2, 2005·Mutation Research·Bennett Van HoutenCaroline Kisker
Feb 16, 2000·Gene·D P Batty, R D Wood
Sep 4, 2010·Antioxidants & Redox Signaling·Jennifer N Earley, John J Turchi
Dec 12, 2012·Antioxidants & Redox Signaling·Joost P M MelisMirjam Luijten
Dec 23, 2004·Nucleic Acids Research·Regula Muheim-LenzHanspeter Naegeli
Jun 23, 2001·Protein Science : a Publication of the Protein Society·L M IakouchevaE J Ackerman
Jul 26, 2002·Molecular and Cellular Biology·Joyce T Reardon, Aziz Sancar
May 30, 2003·Molecular and Cellular Biology·Ryujiro Hara, Aziz Sancar
Jun 27, 2008·PLoS Biology·Stefanie C WolskiCaroline Kisker
May 21, 2010·Biological Chemistry·Stefanie C WolskiCaroline Kisker
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Wakasugi, A Sancar
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·N Buschta-HedayatH Naegeli
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karen M VasquezPeter M Glazer
Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Irina G MinkoR Stephen Lloyd
Jan 7, 2011·Journal of Nucleic Acids·Rajesh P RastogiRajeshwar P Sinha
Jun 13, 2012·Journal of Biomedicine & Biotechnology·Claudia P Spampinato, Diego F Gomez-Casati
Sep 24, 2016·Quarterly Reviews of Biophysics·Sandra C KochThomas Carell
Jul 19, 2011·Mutation Research·Joost P M MelisHarry van Steeg
Jun 21, 2011·DNA Repair·Hanspeter Naegeli, Kaoru Sugasawa
Apr 7, 2011·Mechanisms of Ageing and Development·Thierry Nouspikel
Jan 6, 2009·Molecular Carcinogenesis·Guliang Wang, Karen M Vasquez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.