Biphasic action of phenylephrine on the Ca(2+)-activated K+ channel of human prostatic smooth muscle cells

Urologia Internationalis
Y KurokawaY Nakaya

Abstract

The elevation of cytosolic Ca2+ ([Ca2+]i) is known to regulate smooth muscle contractility. A physiological concentration of phenylephrine induced the elevation in [Ca2+]i of human prostatic smooth muscle cells; however, contraction of prostatic tissues in vitro needs a higher concentration of phenylephrine than the physiological level. To investigate this discrepancy, we investigated the functional importance of the Ca(2+)-activated K+ channel (KCa channel) of human prostatic smooth muscle cells in phenylephrine-induced contraction. Using the patch-clamp technique, the KCa channel of human prostatic smooth muscle cells was activated by phenylephrine at a physiological concentration (10(-7)-10(-5) M) but was inhibited at a higher concentration (10(-4)-10(-3) M). Phenylephrine (10(-3) M) also inhibited the KCa channel which was activated by 10 microM A23187, a calcium ionophore. Similar inhibition was obtained with 1 microM phorbol 12-myristate 13-acetate, an activator of protein kinase C (C-kinase). Both inhibitions were reversed by subsequent application of 1 nM staurosporine, a protein kinase inhibitor. These results suggested that C-kinase mediated the phenylephrine-induced inhibition of the KCa channel. In this study, a phy...Continue Reading

Citations

Oct 3, 2002·Cellular Signalling·Anna-Louise M CookJohn M Haynes
Oct 22, 1998·International Journal of Urology : Official Journal of the Japanese Urological Association·Y KurokawaY Nakaya
Jan 13, 2006·The Journal of Urology·Richard J LangBetty Exintaris
Aug 18, 2004·The Journal of Urology·Richard J LangBetty Exintaris
May 30, 2007·British Journal of Pharmacology·D-T T NguyenB Exintaris

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