Abstract
We developed a biphasic culture system consisting of 4 ml of brucella agar (BA) and 6 ml of brucella broth (BB) in 25-cm2 tissue culture flasks, which were incubated in air (BB/BAa) or in a gas mixture of 5% O2, 10% CO2, and 85% N2 (BB/BAg). These media were also used with a supplement consisting of ferrous sulfate, sodium metabisulfite, and sodium pyruvate and incubated as above (FB/FAa and FB/FAg, respectively). Highly satisfactory growth of Campylobacter jejuni 301 was obtained with all medium-gas phase combinations provided that the number of viable cells in the inoculum was large (greater than or equal to 10(6)/ml). The use of FB/FAa permitted the inoculum to be reduced to 100 cells per ml. With an adjusted gas phase (BB/BAg and FB/FAg), near-optimal growth was obtained from an inoculum of 1 to 10 cells per ml. Under most of these conditions the generation time was approximately 90 min. During the logarithmic growth phase, the cells retained their typical spiral morphology and high motility. These media also proved to be highly satisfactory for the cultivation of fresh isolates as well as other stock strains of Campylobacter. When the broth phase of the cultures, after addition of 15% glycerol, was quickly frozen and maint...Continue Reading
References
Mar 1, 1975·Infection and Immunity·E C McCoyA J Winter
Mar 18, 1978·Lancet·S LauwersJ P Butzler
Jan 1, 1979·Canadian Journal of Microbiology·P S HoffmanR M Smibert
Aug 1, 1979·Annals of Internal Medicine·M J BlaserW L Wang
Apr 1, 1975·Journal of Bacteriology·E C McCoyA J Winter
Jan 1, 1975·Journal of Clinical Microbiology·M N Hwang, G M Ederer
Mar 1, 1973·The Journal of Pediatrics·J P ButzlerF Dehaen
Apr 1, 1981·Journal of Clinical Microbiology·O P DailyC R Lissner
Nov 1, 1980·Journal of Clinical Pathology·M B Skirrow, J Benjamin
Apr 1, 1980·Journal of Clinical Microbiology·S M Harvey
May 1, 1980·The Journal of Infectious Diseases·M J BlaserW L Wang
Sep 1, 1980·The Journal of Infectious Diseases·A Svedhem, B Kaijser
Dec 1, 1980·The Journal of Hygiene·M B Skirrow, J Benjamin
Jan 1, 1958·Journal of Bacteriology·E A TYRRELLP GERHARDT
May 1, 1960·Journal of Bacteriology·C R MANCLARK, M J PICKETT
Citations
Jan 1, 1987·The Journal of Applied Bacteriology·A P Moran, M E Upton
Apr 10, 1999·Letters in Applied Microbiology·C ThomasM Mabey
Jun 14, 2001·Journal of Food Protection·H YangM G Johnson
Jan 15, 2008·Applied and Environmental Microbiology·Anne N ReidAlain Stintzi
Feb 1, 1986·The Journal of Applied Bacteriology·A P Moran, M E Upton
Jan 5, 2006·Applied and Environmental Microbiology·Hong T T NguyenChristopher A Miles
Jan 2, 2009·Microbiology·John A WrightDuncan J Maskell
Mar 1, 1996·Letters in Applied Microbiology·J S DicksonA L Baetz
Sep 18, 2007·Applied and Environmental Microbiology·Diana Axelsson-OlssonBjörn Olsen
Mar 1, 1984·Journal of Clinical Microbiology·H N WestfallE Weiss
Sep 1, 1986·Applied and Environmental Microbiology·D M Rollins, R R Colwell
Oct 1, 1986·Applied and Environmental Microbiology·H N WestfallE Weiss
Jan 1, 1992·International Journal of Food Microbiology·R R BeumerF M Rombouts
Oct 1, 1994·Infection and Immunity·Y L WuW M Ching
Jun 1, 1991·Journal of Clinical Microbiology·H E BokH H Crewe-Brown
Jul 1, 1986·Infection and Immunity·E McSweegan, R I Walker
Feb 1, 1985·Journal of Clinical Microbiology·L K NgD E Taylor
Oct 4, 2000·Journal of Clinical Microbiology·R TolcinG W Procop
Oct 1, 1986·Infection and Immunity·L H FieldL J Berry
Feb 1, 1986·Applied and Environmental Microbiology·M J Blaser, H J Cody
Sep 1, 1992·Infection and Immunity·C L PickettS S Jusuf
Sep 1, 1989·Journal of Clinical Microbiology·M L Hänninen
Feb 1, 1992·Journal of Comparative Pathology·M R AldertonP J Coloe